Frequency of serological non-responders and false-negative RT-PCR leads to SARS-CoV-2 testing: a population-based research
Goals The sensitivity of molecular and serological strategies for COVID-19 testing in an epidemiological setting isn’t effectively described. The intention of the research was to find out the frequency of detrimental RT-PCR outcomes at first scientific presentation in addition to detrimental serological outcomes after a follow-up of at the least Three weeks.
Strategies Amongst all sufferers seen for suspected COVID-19 in Liechtenstein (n=1921), we included initially RT-PCR constructive index sufferers (n=85) in addition to initially RT-PCR detrimental (n=66) for follow-up with SARS-CoV-2 antibody testing.
Antibodies had been detected with seven completely different commercially obtainable immunoassays. Frequencies of detrimental RT-PCR and serology leads to people with COVID-19 had been decided and in comparison with these noticed in a validation cohort of Swiss sufferers (n=211).
Outcomes Amongst COVID-19 sufferers in Liechtenstein, false-negative RT-PCR at preliminary presentation was seen in 18% (12/66), whereas detrimental serology in COVID-19 sufferers was 4% (3/85). The validation cohort confirmed related frequencies: 2/66 (3%) for detrimental serology, and 16/155 (10%) for false detrimental RT-PCR. COVID-19 sufferers with detrimental follow-up serology tended to have an extended illness period (p=0.05) and extra scientific signs than different sufferers with COVID-19 (p<0.05).
The antibody titer from quantitative immunoassays was positively related to the variety of illness signs and illness period (p<0.001). Conclusions RT-PCR at preliminary presentation in sufferers with suspected COVID-19 can miss contaminated sufferers. Antibody titers of SARS-CoV-2 assays are linked to the variety of illness signs and the period of illness. One in 25 sufferers with RT-PCR-positive COVID-19 doesn’t develop antibodies detectable with incessantly employed and commercially obtainable immunoassays.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA kit for detection of Versican from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A polyclonal antibody for detection of Versican from Human. This Versican antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Versican at AA rangle: 510-590
Description: A polyclonal antibody for detection of Versican from Human. This Versican antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Versican at AA rangle: 510-590
Description: A polyclonal antibody for detection of Versican from Human. This Versican antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Versican at AA rangle: 510-590
Description: A polyclonal antibody for detection of Versican from Human, Mouse, Rat. This Versican antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Versican protein at amino acid sequence of 2530-2580
Description: A polyclonal antibody for detection of Versican from Human, Mouse, Rat. This Versican antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Versican protein at amino acid sequence of 2530-2580
Description: A polyclonal antibody for detection of Versican from Human, Mouse, Rat. This Versican antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Versican protein at amino acid sequence of 2530-2580
Description: Quantitativesandwich ELISA kit for measuring Human versican/PG-M/PG-350 in samples from serum, cell culture supernates, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Human versican/PG-M/PG-350 in samples from serum, cell culture supernates, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Versican core protein in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Canine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A monoclonal antibody from clone S351-23 against Mouse Versican. The host species for the production of this antibody is Mouse. The antigen used for immunization is Mouse Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for Versican is not conjugated.
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When ought to clinicians repeat SARS-CoV-2 RT-PCR?: Repeat PCR testing concentrating on sufferers with pulmonary CT findings suggestive of COVID-19
Actual-time reverse transcription polymerase chain response (RT-PCR) testing for SARS-CoV-2 is typically repeated when clinicians suspect a false-negative consequence, however the circumstances below which repeated RT-PCR testing is warranted stay unclear. We evaluated the follow of repeat RT-PCR testing for SARS-CoV-2 in 45 sufferers who retested after an preliminary detrimental PCR check. Of those, the analysis of coronavirus illness (COVID-19) was confirmed in 4 sufferers with typical chest computed tomography (CT) findings, and one affected person with out typical CT findings in whom the check consequence was strongly suspected to be false constructive. We advocate repeat RT-PCR just for sufferers with typical CT findings of COVID-19.
A survey of gastrointestinal nematode species in pink deer (Cervus elaphus) farms in New Zealand utilizing PCR
Gastrointestinal nematodes are recognised as an animal well being situation for farmed pink deer. The intention of this research was to discover the vary of species infecting farmed deer herds and their farm-level prevalence in New Zealand. Faecal samples had been collected from 12-24-month-old deer (n = 6-26; imply 19) on 59 farms positioned within the North (n = 25) and South (n = 34) Islands. Sub-samples of faeces had been pooled by farm and cultured to recuperate third stage larvae. Twenty 4 larvae had been randomly chosen and recognized to species utilizing a multiplex PCR (complete = 1217 larvae).
At farm-level probably the most prevalent nematodes had been Oesophagostomum venulosum 83% (n = 49) and the deer-specific nematodes within the subfamily Ostertagiinae (=Ostertagia-type) together with, Spiculoptera asymmetrica 73% (n = 43), Ostertagia leptospicularis 47% (n = 28), Spiculoptera spiculoptera 47% (n = 28). The just lately recognized Trichostrongylus askivali was current on 32% (n = 19) of the farms and Oesophagostomum sikae on 17% (n = 10).
Within the evaluation of the whole variety of larvae recognized, the proportion was in related order, 45% (n = 548) had been O. venulosum, 14% (n = 173) S. asymmetrica, 10% (n = 124) S. spiculoptera, 9% (n = 114) O. leptospicularis, T. askivali, 3% (n = 40) and solely 2% had been O. sikae (n = 20). This research is the primary to point out the farm-level prevalence of nematode species in deer in New Zealand and the primary to make use of PCR as a diagnostic software.
It supplies knowledge per cross-infection from sheep/cattle to deer, and offered tentative insights into the proportions of the primary GIN species throughout the deer inhabitants together with O. sikae and T. askivali which have solely just lately been recognized in New Zealand.
Description: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Description: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Description: CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, alpha5beta1 integrin and alphaIIbbeta3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
Description: CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, alpha5beta1 integrin and alphaIIbbeta3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
Description: sCD40 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 155 amino acids (112-260 a.a.) and having a molecular mass of 17.2kDa (Migrates at 18-28kDa on SDS-PAGE under reducing conditions).;sCD40 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Description: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Description: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40L is an 18kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant murine CD40L is a soluble 16.4 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Description: Human CD40 Ligand ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant CD40 Ligand in a sandwich ELISA format within the range of 50-3,000 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay CD40 Ligand in approximately 1,500 ELISA plate wells.
Description: Enzyme-linked immunosorbent assay kit for quantification of Bovine CD40 ligand in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: CD40, a member of the TNF receptor superfamily, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40 Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant Human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Description: A sandwich ELISA for quantitative measurement of Mouse CD30 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse CD30 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse CD30 Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human CellExp? CD40 Ligand / TNFSF5, Human Recombinant
Description: CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
Recombinant Human CD40 Ligand/CD40L/TNFSF5 (E. coli)
Description: Recombinant FLT-3 Ligand is a disulfide-linked monomer protein consisting of 164 amino acid residues, and migrates as an approximately 32 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse FLT-3 ligand mature chain was expressed in E. coli.
Description: Recombinant FLT-3 Ligand is a disulfide-linked monomer protein consisting of 164 amino acid residues, and migrates as an approximately 32 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse FLT-3 ligand mature chain was expressed in E. coli.
Human / Rhesus macaque CD40 Ligand Protein (Met 113-Leu 261) [Fc]
Strategies Amongst all sufferers seen for suspected COVID-19 in Liechtenstein (n=1921), we included initially RT-PCR constructive index sufferers (n=85) in addition to initially RT-PCR detrimental (n=66) for follow-up with SARS-CoV-2 antibody testing.
Antibodies had been detected with seven completely different commercially obtainable immunoassays. Frequencies of detrimental RT-PCR and serology leads to people with COVID-19 had been decided and in comparison with these noticed in a validation cohort of Swiss sufferers (n=211).
Outcomes Amongst COVID-19 sufferers in Liechtenstein, false-negative RT-PCR at preliminary presentation was seen in 18% (12/66), whereas detrimental serology in COVID-19 sufferers was 4% (3/85). The validation cohort confirmed related frequencies: 2/66 (3%) for detrimental serology, and 16/155 (10%) for false detrimental RT-PCR. COVID-19 sufferers with detrimental follow-up serology tended to have an extended illness period (p=0.05) and extra scientific signs than different sufferers with COVID-19 (p<0.05).
The antibody titer from quantitative immunoassays was positively related to the variety of illness signs and illness period (p<0.001). Conclusions RT-PCR at preliminary presentation in sufferers with suspected COVID-19 can miss contaminated sufferers. Antibody titers of SARS-CoV-2 assays are linked to the variety of illness signs and the period of illness. One in 25 sufferers with RT-PCR-positive COVID-19 doesn’t develop antibodies detectable with incessantly employed and commercially obtainable immunoassays.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Versican (VS) in serum, plasma and other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Versican (VS) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Versican (VS) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: A sandwich ELISA kit for detection of Versican from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A polyclonal antibody for detection of Versican from Human. This Versican antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Versican at AA rangle: 510-590
Description: A polyclonal antibody for detection of Versican from Human. This Versican antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Versican at AA rangle: 510-590
Description: A polyclonal antibody for detection of Versican from Human. This Versican antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human Versican at AA rangle: 510-590
Description: A polyclonal antibody for detection of Versican from Human, Mouse, Rat. This Versican antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Versican protein at amino acid sequence of 2530-2580
Description: A polyclonal antibody for detection of Versican from Human, Mouse, Rat. This Versican antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Versican protein at amino acid sequence of 2530-2580
Description: A polyclonal antibody for detection of Versican from Human, Mouse, Rat. This Versican antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human Versican protein at amino acid sequence of 2530-2580
Description: Quantitativesandwich ELISA kit for measuring Human versican/PG-M/PG-350 in samples from serum, cell culture supernates, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Human versican/PG-M/PG-350 in samples from serum, cell culture supernates, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Versican core protein in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A competitive ELISA for quantitative measurement of Canine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Versican in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A monoclonal antibody from clone S351-23 against Mouse Versican. The host species for the production of this antibody is Mouse. The antigen used for immunization is Mouse Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein. The antibody is tested and validated for WB, ICC/IF assays with the following recommended dilutions: WB (1:1000), ICC/IF (1:100). This MAb for Versican is not conjugated.
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When ought to clinicians repeat SARS-CoV-2 RT-PCR?: Repeat PCR testing concentrating on sufferers with pulmonary CT findings suggestive of COVID-19
Actual-time reverse transcription polymerase chain response (RT-PCR) testing for SARS-CoV-2 is typically repeated when clinicians suspect a false-negative consequence, however the circumstances below which repeated RT-PCR testing is warranted stay unclear. We evaluated the follow of repeat RT-PCR testing for SARS-CoV-2 in 45 sufferers who retested after an preliminary detrimental PCR check. Of those, the analysis of coronavirus illness (COVID-19) was confirmed in 4 sufferers with typical chest computed tomography (CT) findings, and one affected person with out typical CT findings in whom the check consequence was strongly suspected to be false constructive. We advocate repeat RT-PCR just for sufferers with typical CT findings of COVID-19.
A survey of gastrointestinal nematode species in pink deer (Cervus elaphus) farms in New Zealand utilizing PCR
Gastrointestinal nematodes are recognised as an animal well being situation for farmed pink deer. The intention of this research was to discover the vary of species infecting farmed deer herds and their farm-level prevalence in New Zealand. Faecal samples had been collected from 12-24-month-old deer (n = 6-26; imply 19) on 59 farms positioned within the North (n = 25) and South (n = 34) Islands. Sub-samples of faeces had been pooled by farm and cultured to recuperate third stage larvae. Twenty 4 larvae had been randomly chosen and recognized to species utilizing a multiplex PCR (complete = 1217 larvae).
At farm-level probably the most prevalent nematodes had been Oesophagostomum venulosum 83% (n = 49) and the deer-specific nematodes within the subfamily Ostertagiinae (=Ostertagia-type) together with, Spiculoptera asymmetrica 73% (n = 43), Ostertagia leptospicularis 47% (n = 28), Spiculoptera spiculoptera 47% (n = 28). The just lately recognized Trichostrongylus askivali was current on 32% (n = 19) of the farms and Oesophagostomum sikae on 17% (n = 10).
Within the evaluation of the whole variety of larvae recognized, the proportion was in related order, 45% (n = 548) had been O. venulosum, 14% (n = 173) S. asymmetrica, 10% (n = 124) S. spiculoptera, 9% (n = 114) O. leptospicularis, T. askivali, 3% (n = 40) and solely 2% had been O. sikae (n = 20). This research is the primary to point out the farm-level prevalence of nematode species in deer in New Zealand and the primary to make use of PCR as a diagnostic software.
It supplies knowledge per cross-infection from sheep/cattle to deer, and offered tentative insights into the proportions of the primary GIN species throughout the deer inhabitants together with O. sikae and T. askivali which have solely just lately been recognized in New Zealand.
Description: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Description: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Description: CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members.CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In gen
