PCR Assays for Monitoring of some Fusarium Species Inflicting Fusarium Head Blight in Cereals
Quadruple adverse SARS-CoV-2-PCR: nonetheless COVID-19 pneumonia!
Medical historical past and scientific findings: A 78-year-old man fell in poor health with weak point, coughing and fever 19 days after a cruise in early April 2020 and was admitted four days later with growing shortness of breath.
Examination and analysis: On admission, the affected person had subfebrile temperatures, train dyspnea, and right-basal rales. CRP was reasonably elevated and oxygen saturation was barely diminished. Thoracic CT confirmed bilateral ground-glass infiltrates. Instantly after the cruise a nasopharyngeal swab was adverse for SARS-CoV-2.
Remedy and course: Because of the truth that the affected person’s asymptomatic spouse had been examined constructive for SARS-CoV-2 instantly after coming back from the cruise, we suspected COVID-19 illness and admitted the affected person to our isolation ward. Two nasopharyngeal swabs and bronchial lavage yielded adverse outcomes for SARS-CoV-2. Lastly, suspected COVID-19 analysis was verified serologically.
Conclusion: In case of a excessive diploma of scientific suspicion together with typical findings of thoracic imaging, the suspected analysis COVID-19 illness must be maintained even in case of a number of adverse SARS-CoV-2-PCR. Seroconversion happens just a few days to 2 weeks after the onset of signs and can be utilized to substantiate the analysis.
Anamnese und klinischer befund: Ein 78-jähriger Mann erkrankte Anfang April 2020 19 Tage nach einer Kreuzfahrt mit Schwäche, Husten und Fieber und wurde four Tage später mit zunehmender Luftnot vorstellig.
Untersuchung und diagnose: Bei Aufnahme bestanden subfebrile Temperaturen, Belastungsdyspnoe und rechtsbasale Rasselgeräusche. Das CRP conflict mäßig erhöht und die Sauerstoffsättigung gering reduziert. Im Thorax-CT fanden sich bilaterale Milchglasinfiltrate. Unmittelbar nach der Kreuzfahrt conflict ein nasopharyngealer Abstrich auf SARS-CoV-2 negativ.
Therapie und verlauf: In Kenntnis der Tatsache, dass die symptomfreie Ehefrau des Patienten unmittelbar nach Rückkehr von der Kreuzfahrt positiv auf SARS-CoV-2 getestet worden conflict, gingen wir von einer COVID-19-Erkrankung aus und hospitalisierten den Patienten. Zwei nasopharyngeale Abstriche und die Bronchiallavage ergaben adverse Befunde für SARS-CoV-2, die Diagnose COVID-19 wurde letztlich serologisch verifiziert.
Folgerung: Bei hochgradigem klinisch-anamnestischem Verdacht in Verbindung mit typischen Befunden der Thorax-Bildgebung sollte die Verdachtsdiagnose COVID-19 auch im Falle mehrfach negativer SARS-CoV-2-PCR aufrechterhalten bleiben. Die Serokonversion tritt einige Tage bis 2 Wochen nach Symptombeginn auf und kann im Verlauf zur endgültigen Diagnosesicherung genutzt werden.
Anti-Human IgG |
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| DB-173-0.1 | DB Biotech | 100 μl | EUR 212 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-0.2 | DB Biotech | 200 μl | EUR 298 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-0.5 | DB Biotech | 500 μl | EUR 384 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-1 | DB Biotech | 1 ml | EUR 613 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-173-RTU-15 | DB Biotech | 15 ml | EUR 355 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB-173-RTU-7 | DB Biotech | 7 ml | EUR 231 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB-174-0.1 | DB Biotech | 100 μl | EUR 212 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-0.2 | DB Biotech | 200 μl | EUR 298 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-0.5 | DB Biotech | 500 μl | EUR 384 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-1 | DB Biotech | 1 ml | EUR 613 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated |
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Anti-Human IgG |
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| DB-174-RTU-15 | DB Biotech | 15 ml | EUR 355 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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Anti-Human IgG |
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| DB-174-RTU-7 | DB Biotech | 7 ml | EUR 231 |
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Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use) |
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anti-human RecQL4 |
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| AR05-PA0007 | Abfrontier | 100 ul | EUR 334 |
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Description: Rabbit polyclonal to human RecQL4 |
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anti-human Albumin |
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| LF-PA10001 | Abfrontier | 100 ug | EUR 403 |
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Description: Rabbit polyclonal to human Albumin |
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anti-human CCR1 |
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| 20R-3028 | Fitzgerald | 200 ug | EUR 587 |
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Description: Goat anti-human CCR1 antibody |
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Anti-Procalcitonin antibody *Mouse anti-human, monoclonal * |
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| V100125 | AAT Bioquest | 50 ug | EUR 306 |
Anti-Procalcitonin antibody *Mouse anti-human, monoclonal * |
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| V100126 | AAT Bioquest | 1 mg | EUR 393 |
Goat anti-Human anti-thrombin polyclonal antibody |
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| CABT-L487 | Creative Diagnostics | 500ug | EUR 715 |
Sheep anti-Human anti-thrombin polyclonal antibody |
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| CABT-L488 | Creative Diagnostics | 500ug | EUR 663 |
Sheep anti-Human anti-thrombin polyclonal antibody |
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| CABT-L489 | Creative Diagnostics | 100ug | EUR 663 |
Sheep anti-Human anti-thrombin polyclonal antibody |
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| CABT-L490 | Creative Diagnostics | 100ug | EUR 663 |
Sheep anti-Human anti-thrombin polyclonal antibody |
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| CABT-L491 | Creative Diagnostics | 100ug | EUR 663 |
Human anti AMPH(anti amphiphysin) ELISA Kit |
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| EH2621 | FN Test | 96T | EUR 524.1 |
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Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml |
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Human anti-TPO(anti-thrombopoietin) ELISA Kit |
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| EH4147 | FN Test | 96T | EUR 524.1 |
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Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens ;Sensitivity: 0.938 ng/ml |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5647.8 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 552.76 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 746.8 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| AEA465Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3060.6 |
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Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids. |
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Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit |
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| 4-AEA465Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species. |
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ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody) |
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| ELK8071 | ELK Biotech | 1 plate of 96 wells | EUR 432 |
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Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
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Anti-human EPO Antibody |
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| RP1004 | BosterBio | 100ug/vial | EUR 334 |
Anti-Human Ki67 Antibody |
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| M00254-3 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Human Ki67 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat. |
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Anti-Human IBA1 Antibody |
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| M01394-2 | BosterBio | 100ul | EUR 397 |
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Description: Rabbit Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human. |
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Anti-Human IBA1 Antibody |
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| M01394-3 | BosterBio | 100ul | EUR 397 |
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Description: Chicken Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human. |
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Anti-Human IgG:HRP Conjugate |
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| F163 | Cygnus Technologies | 12 ml | EUR 488 |
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Description: Anti-Human IgG:HRP Conjugate by Cygnus Technologies is available in Europe via Gentaur. |
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Goat, anti-human IgA |
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| IM140 | Cygnus Technologies | 1 ml | EUR 359 |
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Description: Goat, anti-human IgA by Cygnus Technologies is available in Europe via Gentaur. |
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Goat, anti-human IgD |
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| IM141 | Cygnus Technologies | 1 ml | EUR 359 |
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Description: Goat, anti-human IgD by Cygnus Technologies is available in Europe via Gentaur. |
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Goat, anti-human IgE |
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| IM142 | Cygnus Technologies | 1 ml | EUR 359 |
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Description: Goat, anti-human IgE by Cygnus Technologies is available in Europe via Gentaur. |
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Goat, anti-human IgM |
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| IM144 | Cygnus Technologies | 1 ml | EUR 359 |
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Description: Goat, anti-human IgM by Cygnus Technologies is available in Europe via Gentaur. |
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Anti-Human IgG1:HRP |
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| IM151 | Cygnus Technologies | 1 ml | EUR 322 |
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Description: Anti-Human IgG1:HRP by Cygnus Technologies is available in Europe via Gentaur. |
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Anti-Human IgG2:HRP |
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| IM152 | Cygnus Technologies | 1 ml | EUR 322 |
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Description: Anti-Human IgG2:HRP by Cygnus Technologies is available in Europe via Gentaur. |
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Anti-Human IgG3:HRP |
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| IM153 | Cygnus Technologies | 1 ml | EUR 322 |
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Description: Anti-Human IgG3:HRP by Cygnus Technologies is available in Europe via Gentaur. |
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Anti-Human IgG4:HRP |
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| IM154 | Cygnus Technologies | 1 ml | EUR 359 |
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Description: Anti-Human IgG4:HRP by Cygnus Technologies is available in Europe via Gentaur. |
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Sheep, Anti-human IgG4 |
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| IM154U | Cygnus Technologies | 1 ml | EUR 506 |
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Description: Sheep, Anti-human IgG4 by Cygnus Technologies is available in Europe via Gentaur. |
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Rabbit Anti Human IgG |
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| E61I00101 | EnoGene | 100ug | EUR 343 |
Goat Anti Human IgG |
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| E61I00201 | EnoGene | 1mg | EUR 274 |
Goat Anti Human IgM |
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| E61I00401 | EnoGene | 100ug | EUR 343 |
Mouse Anti Human IgM |
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| E61I00402 | EnoGene | 100ug | EUR 343 |
Goat anti human IgA |
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| E61I01201 | EnoGene | 1mg | EUR 611 |
Mouse anti human IgA |
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| E61I02101 | EnoGene | 100ug | EUR 343 |
Goat anti human IgA |
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| E61I02102 | EnoGene | 100ug | EUR 343 |
Mouse anti human IgE |
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| E61I02201 | EnoGene | 100ug | EUR 343 |
Anti-Human IgG antibody |
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| STJ140086 | St John's Laboratory | 200 µg | EUR 219 |
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Description: Goat polyclonal antibody to human IgG. IgG is a monomeric immunoglobulin, with two heavy chains and two light chains. IgG can be found in blood and is the most abundant immunoglobulin, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses (IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%)) that can bind to many types of pathogens, protecting the body against them by complement activation, opsonization for phagocytosis and neutralisation of their toxins. |
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Anti-human Insulin antibody |
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| STJ15100014 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of human Insulin in ELISA. |
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Anti-human Insulin antibody |
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| STJ15100015 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human Insulin in ELISA. |
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Anti-human EBI3 antibody |
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| STJ15100091 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IL-27 in immunoassays such as ELISA and ELISpot. |
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Anti-human Perforin antibody |
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| STJ15100095 | St John's Laboratory | 2 mg | EUR 863 |
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Description: These monoclonal antibodies enable sensitive and specific detection of human Perforin in immunoassays such as ELISA and ELISpot. |
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Anti-human Perforin antibody |
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| STJ15100096 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human Perforin in immunoassays such as ELISA and ELISpot. |
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Anti-human CD40 antibody |
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| STJ15100139 | St John's Laboratory | 250 µg | EUR 273 |
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Description: This monoclonal antibody is suitable for studies of CD40 expression on cells and tissue sections and for studies of B-cell activation, proliferation and differentiation. |
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Anti-human CD40 antibody |
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| STJ15100140 | St John's Laboratory | 250 µg | EUR 305 |
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Description: This monoclonal antibody is suitable for studies of CD40 expression on cells and tissue sections. |
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Anti-human CD43 antibody |
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| STJ15100141 | St John's Laboratory | 250 µg | EUR 273 |
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Description: This monoclonal antibody is suitable for studies of T-cell activation, CD43 expression on cells and tissue sections. |
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Anti-human CD43 antibody |
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| STJ15100142 | St John's Laboratory | 250 µg | EUR 305 |
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Description: This monoclonal antibody is suitable for studies of T-cell activation, CD43 expression on cells and tissue sections. |
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Anti-human CD40 antibody |
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| STJ15100143 | St John's Laboratory | 250 µg | EUR 273 |
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Description: This monoclonal antibody is suitable for studies of CD40 expression on cells and tissue sections and for studies of B-cell activation, proliferation and differentiation. |
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Anti-human CD4 antibody |
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| STJ15100144 | St John's Laboratory | 250 µg | EUR 273 |
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Description: This monoclonal antibody is specific for human CD4 and is suitable for immunohistochemistry. |
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Anti-human CD8 antibody |
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| STJ15100145 | St John's Laboratory | 250 µg | EUR 273 |
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Description: This monoclonal antibody is specific for human CD8 and is suitable for immunohistochemistry. |
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Anti-human apoA1 antibody |
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| STJ15100147 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoA1 in immunoassays such as ELISA. The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%. |
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Anti-human apoA1 antibody |
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| STJ15100148 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoA1 in immunoassays such as ELISA. The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%. |
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Anti-human apoH antibody |
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| STJ15100149 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoH in immunoassays such as ELISA. |
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Anti-human apoH antibody |
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| STJ15100150 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoH in immunoassays such as ELISA. |
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Anti-human apoE antibody |
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| STJ15100151 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of human apoE in immunoassays such as ELISA, immunoprecipitation and western blot. |
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Anti-human apoE antibody |
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| STJ15100152 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoE in immunoassays such as ELISA, immunoprecipitation and western blot. |
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Anti-human apoD antibody |
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| STJ15100156 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of human apoD in immunoassays such as ELISA |
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Anti-human apoD antibody |
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| STJ15100157 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human apoD in immunoassays such as ELISA |
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Anti-human apoB antibody |
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| STJ15100158 | St John's Laboratory | 250 µg | EUR 336 |
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Description: These monoclonal antibodies enable sensitive and specific detection of apoB in immunoassays such as ELISA. |
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Anti-human apoB antibody |
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| STJ15100159 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoB in immunoassays such as ELISA and ELISpot. The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.001-0.5%. |
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Anti-human apoB antibody |
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| STJ15100160 | St John's Laboratory | 250 µg | EUR 389 |
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Description: For intracellular staining of LDL, the simultaneous addition of fixative and a non-ionic detergent is recommended. |
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Anti-human apoM antibody |
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| STJ15100161 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of apoM in immunoassays such as ELISA, immunoprecipitation and Western blot. |
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Anti-human apoM antibody |
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| STJ15100162 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human apoM in ELISA. |
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Anti-human IgE antibody |
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| STJ15100170 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody offers sensitive and specific detection of human IgE in immunoassays such as ELISA and ELISpot. |
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Anti-human IgE antibody |
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| STJ15100171 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody offers sensitive and specific detection of human IgE in immunoassays such as ELISA. |
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Anti-human IgE antibody |
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| STJ15100172 | St John's Laboratory | 250 µg | EUR 336 |
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Description: These monoclonal antibodies are recommended for detection of antigen-specific IgE in e.g. indirect ELISA |
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Anti-human IgE antibody |
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| STJ15100173 | St John's Laboratory | 250 µg | EUR 367 |
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Description: These monoclonal antibodies are recommended for detection of human IgE in ELISA and ELISpot. |
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Goat Anti-human IgG |
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| STJ15100177 | St John's Laboratory | 250 µg | EUR 283 |
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Description: Biotinylated goat anti-human IgG enables detection of human IgG in enzyme immunoassays such as indirect ELISA. |
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Goat Anti-human IgA |
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| STJ15100179 | St John's Laboratory | 250 µg | EUR 283 |
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Description: Biotinylated goat anti-human IgA enables detection of human IgA in enzyme immunoassays such as indirect ELISA. |
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Goat Anti-human IgM |
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| STJ15100181 | St John's Laboratory | 250 µg | EUR 283 |
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Description: Biotinylated goat anti-human IgM enables detection of human IgM in enzyme immunoassays such as indirect ELISA. |
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Anti-human IgG antibody |
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| STJ15100182 | St John's Laboratory | 250 µg | EUR 290 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG in immunoassays such as ELISA. It is also suitable as coating antibody in subclass specific ELISpot. |
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Anti-human IgG antibody |
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| STJ15100183 | St John's Laboratory | 250 µg | EUR 290 |
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Description: These monoclonal antibodies enable sensitive and specific detection of human IgG in immunoassays such as ELISpot. |
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Anti-human IgG antibody |
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| STJ15100184 | St John's Laboratory | 250 µg | EUR 326 |
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Description: These monoclonal antibodies enable sensitive and specific detection of human IgG in immunoassays such as ELISA and ELISpot. |
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Anti-human IgG antibody |
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| STJ15100185 | St John's Laboratory | 1 ml | EUR 350 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG in immunoassays such as ELISA. |
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Anti-human IgG1 antibody |
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| STJ15100186 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG1 in e.g. antigen-specific ELISA. |
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Anti-human IgG1 antibody |
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| STJ15100187 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG1 in e.g. antigen-specific ELISA. |
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Anti-human IgG3 antibody |
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| STJ15100188 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG3 in e.g. antigen-specific ELISA. |
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Anti-human IgG3 antibody |
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| STJ15100189 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG3 in e.g. antigen-specific ELISA. |
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Anti-human IgG4 antibody |
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| STJ15100190 | St John's Laboratory | 250 µg | EUR 336 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG4 in ELISA and ELISpot. In ELISpot it is recommended as capture antibody when detecting with biotinylated antigen. |
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Anti-human IgG4 antibody |
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| STJ15100191 | St John's Laboratory | 250 µg | EUR 367 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgG4 in e.g. antigen-specific ELISA. |
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Anti-human IgA antibody |
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| STJ15100192 | St John's Laboratory | 250 µg | EUR 290 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgA in immunoassays such as ELISA and ELISpot. |
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Anti-human IgA antibody |
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| STJ15100193 | St John's Laboratory | 250 µg | EUR 326 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgA in immunoassays such as ELISpot. |
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Anti-human IgA antibody |
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| STJ15100194 | St John's Laboratory | 250 µg | EUR 326 |
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Description: This monoclonal antibody enables sensitive and specific detection of human IgA in immunoassays such as ELISA and ELISpot. |
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Transferring from qPCR to Chip Digital PCR Assays for Monitoring of some Fusarium Species Inflicting Fusarium Head Blight in Cereals
Fusarium Head Blight (FHB) is without doubt one of the main ailments affecting small-grain cereals, worldwide unfold and chargeable for extreme yield and high quality losses yearly. Diagnostic instruments, in a position to observe Fusarium species even within the early levels of an infection, can contribute to mycotoxins’ threat management.
Amongst DNA-based applied sciences for Fusarium detection, qPCR (single and multiplex assays) is at present probably the most utilized technique. Nonetheless, pathogen diagnostics is now enforced by digital PCR (dPCR), a breakthrough expertise that gives ultrasensitive and absolute nucleic acid quantification. In our work, a panel of chip digital PCR assays was developed to quantify Fusarium graminearum, F.culmorum, F. sporotrichioides, F. poae and F. avenaceum.
The primers/probes combos had been evaluated on pure fungal samples with cdPCR approach, compared with the qPCR method. Furthermore, the cdPCR assays had been utilized to quantify Fusarium in durum wheat and oat samples, naturally contaminated or spiked with fungal DNA. For a greater analysis of an infection stage in crops, duplex assays had been developed, in a position to co-amplify each plant and fungal DNA. To the very best of our data, that is the primary research directed to the appliance of digital PCR to Fusarium analysis in crops.
Prognosis of SARS-CoV-2 by RT-PCR Utilizing Totally different Pattern Sources: Evaluate of the Literature
Goal: Essentially the most extensively used diagnostic approach for extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) an infection is real-time reverse transcriptase-polymerase chain response (RT-PCR). It may be completed on totally different samples: nasopharyngeal swabs (NPS) or oropharyngeal swabs (OPS), and self-collected saliva. Nonetheless, adverse findings don’t rule out an infection.
Strategies: A assessment was conceived to debate benefits and limitations of the obtainable diagnostic modalities for nonserologic analysis of SARS-CoV-2 based mostly on RT-PCR; the article additionally proposes some sensible ideas to enhance diagnostic reliability.
Outcomes: A complete of 16 papers (similar to 452 sufferers) of the 56 initially recognized had been included. Many of the papers describe findings from totally different samples obtained in restricted case sequence; comparative research are lacking.
Conclusions: Diagnostic accuracy of NPS and OPS is suboptimal and the danger of contaminated aerosol dispersal shouldn’t be negligible. The SARS-CoV-2 RNA will be present in self-collected saliva specimens of many contaminated sufferers inside 7 to 10 days after symptom onset. There may be an pressing want for comparative trials to outline the diagnostic modality of selection. Enough training and coaching of well being care personnel is necessary.
anti-Anti-ADAMTS15 |
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| YF-PA26950 | Abfrontier | 50 ul | EUR 334 |
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Description: Mouse polyclonal to Anti-ADAMTS15 |
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anti-Anti-APOBEC3F |
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| YF-PA26963 | Abfrontier | 50 ul | EUR 334 |
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Description: Mouse polyclonal to Anti-APOBEC3F |
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anti-Anti-Lgi3 |
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| YF-PA26969 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-Lgi3 |
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anti-Anti-LASS3 |
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| YF-PA26971 | Abfrontier | 50 ul | EUR 334 |
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Description: Mouse polyclonal to Anti-LASS3 |
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anti-Anti-RNF214 |
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| YF-PA27009 | Abfrontier | 50 ul | EUR 334 |
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Description: Mouse polyclonal to Anti-RNF214 |
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anti-Anti-TMIE |
|||
| YF-PA27013 | Abfrontier | 50 ul | EUR 334 |
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Description: Mouse polyclonal to Anti-TMIE |
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anti-Anti-CATSPER3 |
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| YF-PA27044 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-CATSPER3 |
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anti-Anti-DNAJB13 |
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| YF-PA27047 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-DNAJB13 |
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anti-Anti-RECK |
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| YF-PA15627 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-RECK |
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anti-Anti-RECK |
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| YF-PA15628 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-RECK |
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anti-Anti-RECK |
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| YF-PA15629 | Abfrontier | 100 ug | EUR 403 |
|
Description: Rabbit polyclonal to Anti-RECK |
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anti-Anti-RAI3 |
|||
| YF-PA16054 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-RAI3 |
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anti-Anti-RAI3 |
|||
| YF-PA16055 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-RAI3 |
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anti-Anti-ZNF670 |
|||
| YF-PA21783 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-ZNF670 |
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anti-Anti-MGC16943 |
|||
| YF-PA21814 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-MGC16943 |
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anti-Anti-SAT2 |
|||
| YF-PA21815 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-SAT2 |
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anti-Anti-STK11IP |
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| YF-PA21878 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-STK11IP |
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anti-Anti-LY108 |
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| YF-PA21890 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-LY108 |
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anti-Anti-MDH1B |
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| YF-PA22189 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-MDH1B |
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anti-Anti-ZDHHC19 |
|||
| YF-PA22203 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-ZDHHC19 |
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anti-Anti-UGT3A1 |
|||
| YF-PA22231 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-UGT3A1 |
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anti-Anti-RALYL |
|||
| YF-PA22261 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-RALYL |
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anti-Anti-BTBD14A |
|||
| YF-PA22262 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-BTBD14A |
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anti-Anti-KLC3 |
|||
| YF-PA22405 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-KLC3 |
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anti-Anti-UPP2 |
|||
| YF-PA22477 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-UPP2 |
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anti-Anti-SCFD2 |
|||
| YF-PA22491 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-SCFD2 |
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anti-Anti-DNAJB8 |
|||
| YF-PA22588 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-DNAJB8 |
|||
anti-Anti-ZUFSP |
|||
| YF-PA22749 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-ZUFSP |
|||
anti-Anti-NUDT8 |
|||
| YF-PA22826 | Abfrontier | 50 ul | EUR 363 |
|
Description: Mouse polyclonal to Anti-NUDT8 |
|||
anti-Anti-NUDT8 |
|||
| YF-PA22827 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to Anti-NUDT8 |
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anti-Anti-SYBL1 |
|||
| YF-PA24797 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-SYBL1 |
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anti-Anti-TAF2 |
|||
| YF-PA24803 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-TAF2 |
|||
anti-Anti-TM4SF2 |
|||
| YF-PA24859 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-TM4SF2 |
|||
anti-Anti-TRPC6 |
|||
| YF-PA24902 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-TRPC6 |
|||
anti-Anti-Tubby |
|||
| YF-PA24913 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-Tubby |
|||
anti-Anti-UBA52 |
|||
| YF-PA24923 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-UBA52 |
|||
anti-Anti-Cdc14B |
|||
| YF-PA25130 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-Cdc14B |
|||
anti-Anti-SUCLG1 |
|||
| YF-PA25189 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-SUCLG1 |
|||
anti-Anti-NPIP |
|||
| YF-PA25300 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-NPIP |
|||
anti-Anti-PTER |
|||
| YF-PA25306 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-PTER |
|||
anti-Anti-SEP15 |
|||
| YF-PA25331 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-SEP15 |
|||
anti-Anti-PERK |
|||
| YF-PA25342 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-PERK |
|||
anti-Anti-Rab3D |
|||
| YF-PA25362 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-Rab3D |
|||
anti-Anti-MAML1 |
|||
| YF-PA25409 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-MAML1 |
|||
anti-Anti-SLC12A6 |
|||
| YF-PA25449 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-SLC12A6 |
|||
anti-Anti-PREB |
|||
| YF-PA25488 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-PREB |
|||
anti-Anti-ADAMTS7 |
|||
| YF-PA25755 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-ADAMTS7 |
|||
anti-Anti-GABARAPL2 |
|||
| YF-PA25795 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-GABARAPL2 |
|||
anti-Anti-Palladin |
|||
| YF-PA25831 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-Palladin |
|||
anti-Anti-LARG |
|||
| YF-PA25871 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-LARG |
|||
anti-Anti-EID1 |
|||
| YF-PA25927 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-EID1 |
|||
anti-Anti-IL19 |
|||
| YF-PA26093 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-IL19 |
|||
anti-Anti-RPL26L1 |
|||
| YF-PA26137 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-RPL26L1 |
|||
anti-Anti-DUSP13 |
|||
| YF-PA26151 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-DUSP13 |
|||
anti-Anti-Zfp219 |
|||
| YF-PA26153 | Abfrontier | 50 ul | EUR 334 |
|
Description: Mouse polyclonal to Anti-Zfp219 |
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