josephine
september 13, 2020
A Simplified Quantitative Actual-Time PCR Assay for Monitoring SARS-CoV-2 Development in Cell Tradition
Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has contaminated hundreds of thousands inside just some months, inflicting extreme respiratory illness and mortality. Assays to watch SARS-CoV-2 development in vitro rely on time-consuming and dear RNA extraction steps, hampering progress in primary analysis and drug growth efforts. Right here, we developed a simplified quantitative real-time PCR assay that bypasses viral RNA extraction steps and might monitor SARS-CoV-2 development from a small quantity of cell tradition supernatants. As well as, we present that this method is definitely adaptable to quite a few different RNA and DNA viruses.
Utilizing this assay, we screened the actions of a lot of compounds that had been predicted to change SARS-CoV-2 entry and replication in addition to HIV-1-specific medication in a proof-of-concept examine. We discovered that E64D (inhibitor of endosomal proteases cathepsin B and L) and apilimod (endosomal trafficking inhibitor) potently decreased the quantity of SARS-CoV-2 RNA in cell tradition supernatants with minimal cytotoxicity.
Surprisingly, we discovered that the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) equally decreased SARS-CoV-2 RNA ranges in supernatants, suggesting that entry may also be mediated by an alternate pathway. HIV-1-specific inhibitors nevirapine (a nonnucleoside reverse transcriptase inhibitor [NNRTI]), amprenavir (a protease inhibitor), and allosteric integrase inhibitor 2 (ALLINI-2) modestly inhibited SARS-CoV-2 replication, albeit the 50% inhibitory focus (IC50 ) values had been a lot increased than that required for HIV-1.
Taking the info collectively, this simplified assay will expedite primary SARS-CoV-2 analysis, be amenable to mid-throughput screening assays (i.e., drug, CRISPR, small interfering RNA [siRNA], and so forth.), and be relevant to a broad variety of RNA and DNA viruses.
IMPORTANCE Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of the coronavirus illness 2019 (COVID-19) pandemic, is constant to trigger immense respiratory illness and social and financial disruptions.
Standard assays that monitor SARS-CoV-2 development in cell tradition depend on pricey and time-consuming RNA extraction procedures, hampering progress in primary SARS-CoV-2 analysis and growth of efficient therapeutics. Right here, we developed a easy quantitative real-time PCR assay to monitor SARS-CoV-2 development in cell tradition supernatants that doesn’t necessitate RNA extraction and that’s as correct and delicate as present strategies. In a proof-of-concept display screen, we discovered that E64D, apilimod, EIPA, and remdesivir can considerably impede SARS-Cov-2 replication, offering novel perception into viral entry and replication mechanisms.
As well as, we present that this method is definitely adaptable to quite a few different RNA and DNA viruses. This simplified assay will undoubtedly expedite primary SARS-CoV-2 and virology analysis and be amenable to make use of in drug screening platforms to establish therapeutics in opposition to SARS-CoV-2.
learninglabs
Polyclonal Goat anti-GST μ-form
GST-ANTI-2
Detroit R&D
50 uL
EUR 336
Polyclonal Goat anti-GST p-form
GST-ANTI-3
Detroit R&D
50 uL
EUR 336
Chicken Anti-Human alpha 1 anti-trypsin IgG/Y
A1AT12-A
Alpha Diagnostics
100 ul
EUR 578.4
Rabbit Anti-Human p73 alpha antiserum # 2
P73A12-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Human Synuclein-alpha antiserum # 1
SYN11-S
Alpha Diagnostics
100 ul
EUR 548.4
Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure
LHA13-A
Alpha Diagnostics
1 mg
EUR 343.2
Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure
FSHA12-M
Alpha Diagnostics
1 mg
EUR 578.4
Goat Anti-human Chorionic Gonadotropin Alpha (hCG-Alpha) IgG aff pure
HCGA13-A
Alpha Diagnostics
1 mg
EUR 343.2
Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure
TOP2A11-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Mouse Neprilysin-alpha (NEPL-alpha) antiserum
NEPLA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Monoclonal Anti-Human Chorionic Gonadotropin Alpha (hCG-alpha) IgG #1, aff pure
HCGA11-M
Alpha Diagnostics
1 mg
EUR 343.2
Monoclonal Anti-human Chronionic Gonadotropin Alpha (hCG-Alpha) IgG #2, caff pure
HCGA12-M
Alpha Diagnostics
1 mg
EUR 343.2
Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #2
RELMA12-S
Alpha Diagnostics
100 ul
EUR 548.4
Goat Anti-human Alpha fetoprotein (AFP) IgG, pure
AFP13-A
Alpha Diagnostics
1 mg
EUR 781.2
Rabbit Anti-Human p73 alpha/beta antiserum # 1
P73A11-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Human alpha-Tubulin IgG-Biotin Conjugate
TUBA13-BTN
Alpha Diagnostics
100 ul
EUR 562.8
Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate
TUBA13-HRP
Alpha Diagnostics
100 ul
EUR 518.4
Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum
GSTA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Monoclonal anti-human Lutenizing Hormone Alpha (LH-Alpha) IgG, clone 2 aff pure
LHA12-M
Alpha Diagnostics
1 mg
EUR 343.2
Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #1 aff. Pure
TNFA11-M
Alpha Diagnostics
100 ug
EUR 578.4
Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure
TNFA12-M
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Mouse Neprilysin-alpha (NEPL-alpha) IgG (aff pure)
NEPLA11-A
Alpha Diagnostics
100 ug
EUR 578.4
Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 1)
AFP11-M
Alpha Diagnostics
1 mg
EUR 578.4
Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 2)
AFP12-M
Alpha Diagnostics
1 mg
EUR 578.4
Chicken Anti-Human Alpha 2 Macroglobulin (A2M) IgG/Y
A2MG13-A
Alpha Diagnostics
100 ul
EUR 578.4
Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, unlabeled
A1AT11-A
Alpha Diagnostics
100 ul
EUR 489.6
Rabbit Anti-Human p73 alpha/ IgG # 2, aff pure
P73A12-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human HE2 alpha IgG #1, aff pure
HE2A11-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human Synuclein-alpha IgG # 1, aff pure
SYN11-A
Alpha Diagnostics
100 ug
EUR 578.4
Monoclonal anti-human Follicle Stimulating Hormone Alpha (FSH-Alpha), IgG clone 2 aff pure
FSHA11-M
Alpha Diagnostics
1 mg
EUR 578.4
Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) IgG #2, aff. Pure
RELMA12-A
Alpha Diagnostics
100 ug
EUR 578.4
Goat Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure (neutralzing)
TNFA13-A
Alpha Diagnostics
100 ul
EUR 578.4
Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha
RP-675
Alpha Diagnostics
1 ug
EUR 343.2
Goat Anti-Follicle Stimulating Hormone Alpha (FSH-Alpha) IgG aff pure
FSH13-A
Alpha Diagnostics
1 mg
EUR 578.4
monoclonal Anti-Rat Resistin-Like protein alpha (RELM-alpha/FIZZ1) ascites
RELMA14-M
Alpha Diagnostics
100 ul
EUR 578.4
Rabbit Anti-Human Core-binding factor alpha (CBFA1) antiserum #1
CBFA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Sheep Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure
ACT11-A
Alpha Diagnostics
100 ug
EUR 489.6
Monoclonal Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure
ACT12-M
Alpha Diagnostics
100 ug
EUR 578.4
Human Anti-Alpha Fodrin IgG ELISA kit, 96 tests, Quantitative
3300-160-AFG
Alpha Diagnostics
1 kit
EUR 708
Goat Anti-Human IgA (alpha chain sp.) IgG-Biotin Conjugate
10040
Alpha Diagnostics
0.5 mg
EUR 242.4
Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, unlabeled
A2MG11-A
Alpha Diagnostics
100 ul
EUR 416.4
Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, Biotin conjugate
A1AT11-BT
Alpha Diagnostics
100 ul
EUR 489.6
Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, HRP conjugate
A1AT11-HRP
Alpha Diagnostics
100 ul
EUR 489.6
Rabbit Anti-Human p73 alpha/beta IgG # 1, aff pure
P73A11-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human Alpha Defensin 4 (NP-4) antiserum #1
HDEFA41-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Human Alpha Defensin 5 (NP-5),antiserum #1
HDEFA51-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Human Alpha Defensin 6 (NP-6) antiserum #1
HDEFA61-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) Antiserum #1
GAA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Mouse Monoclonal Anti-Human Synuclein-alpha protein IgG, aff pure
SYNRP-84816-M
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-C. perfringens alpha toxin antiserum
CPA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Rat Synuclein-alpha antiserum # 2
SYN12-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #1
RELMA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Rat monoclonal Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) ascites
RELMA13-M
Alpha Diagnostics
100 ul
EUR 578.4
Human Fodrin-alpha (spectrin-alpha) control/blocking peptide
FOD11-P
Alpha Diagnostics
100 ug
EUR 196.8
Rabbit Anti-Human IL-2 receptor alpha, soluble (IL2RsA/CD25) IgG
CD251-A
Alpha Diagnostics
100 ul
EUR 578.4
Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, Biotin conjugate
A2MG11-BT
Alpha Diagnostics
100 ul
EUR 343.2
Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, HRP conjugate
A2MG11-HRP
Alpha Diagnostics
100 ul
EUR 343.2
Chicken Anti-Human Alpha 1 acid glycoprotein (A1AG) IgG/Y, unlabeled
A1AG12-A
Alpha Diagnostics
100 ul
EUR 578.4
Monoclonal Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein, ascites
A1AG12-M
Alpha Diagnostics
100 ul
EUR 578.4
Monoclonal Anti-Human Lutenizing Hormone (LH), alpha chain IgG, aff pure
LHA14-M
Alpha Diagnostics
1 mg
EUR 343.2
Rabbit Anti-Human GABAa Receptor alpha 2 (GAA2) IgG, aff. Pure
GAA21-A
Alpha Diagnostics
100 ug
EUR 578.4
Monoclonal Anti-Human/mouse/rat HSP90 alpha/beta IgG, aff pure
HSP901-M
Alpha Diagnostics
100 ul
EUR 578.4
human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide
TOP2A11-P
Alpha Diagnostics
100 ug
EUR 196.8
Goat Anti-Chicken IgA (alpha chain)-HRP conjugate
60220
Alpha Diagnostics
0.5 ml
EUR 255.6
Goat Anti-Chicken IgA (alpha chain)-FITC Conjugate
60230
Alpha Diagnostics
0.5 ml
EUR 255.6
Goat Anti-Chicken IgA (alpha chain)-biotin conjugate
60240
Alpha Diagnostics
0.5 ml
EUR 270
Goat Anti-Rabbit IgA (alpha chain)-HRP conjugate
20029
Alpha Diagnostics
0.5 ml
EUR 315.6
Rabbit Anti-Goat IgA (alpha chain)-HRP conjugate
30120
Alpha Diagnostics
0.5 ml
EUR 255.6
Rabbit Anti-Goat IgA (alpha chain)-FITC conjugate
30130
Alpha Diagnostics
0.5 ml
EUR 255.6
Monoclonal Anti-Bovine S100 alpha ab # 2, ascites
S1005-M
Alpha Diagnostics
100 ul
EUR 578.4
Human Alpha-Thrombin (human plasma), purified
TBNA16-N-100
Alpha Diagnostics
100 ug
EUR 196.8
Rabbit Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) IgG#1, aff. pure
RELMA11-A
Alpha Diagnostics
100 ug
EUR 578.4
Monoclonal Anti-Human Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG
FETA13-M
Alpha Diagnostics
100 ug
EUR 578.4
Sheep Anti-Human Serum Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG
FETA15-A
Alpha Diagnostics
100 ul
EUR 489.6
Rabbit Anti-Human interleukin 13 receptor, alpha 2 (IL13RA2) IgG (aff pure)
AB-23040-A
Alpha Diagnostics
100ug
EUR 578.4
Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, unlabeled
A1AG11-A
Alpha Diagnostics
100 ul
EUR 489.6
Mouse Monoclonal Anti-Human Luteinizing Hormone (LH), alpha chain IgG, aff pure
LH13-M
Alpha Diagnostics
1 mg
EUR 343.2
Monoclonal Anti-human Lutenizing Hormone (LH- Alpha) IgG, clone 1 aff pure
LHA11-M
Alpha Diagnostics
1 mg
EUR 343.2
Rabbit Anti-Human Alpha Defensin 4 (NP-4), IgG #1, aff pure
HDEFA41-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human Alpha Defensin 5 (NP-5),, IgG #1, aff pure
HDEFA51-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human Alpha Defensin 6 (NP-6) IgG #1, aff pure
HDEFA61-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) IgG #1, aff pure
GAA11-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human/Rat GABAa Receptor alpha 3 (GAA3) IgG, aff pure
GAA31-A
Alpha Diagnostics
100 ug
EUR 578.4
Rabbit Anti-Human/mouse/rat HSP90 alpha (hsp90AB1) peptide IgG, aff pure
HSP903-A
Alpha Diagnostics
100 ul
EUR 578.4
Rabbit Anti-Human PUMA-alpha (p53-upregulated modulator of apoptosis) antiserum #1
PUMA11-S
Alpha Diagnostics
100 ul
EUR 548.4
Rabbit Anti-Human PUMA-alpha (p53-upregulated modulator of apoptosis) antiserum #1
PUMA12-S
Alpha Diagnostics
100 ul
EUR 548.4
Alpha 1-Antichymotrypsin , Human Plasma
A1CT15-N-100
Alpha Diagnostics
100 ug
EUR 196.8
Recombinant (E.Coli) Human Thyrostimulin Alpha
RP-595
Alpha Diagnostics
10 ug
EUR 343.2
Recombinant purified, human Interleukin-1 alpha (IL-1 alpha), active
IL1A15-R-10
Alpha Diagnostics
10 ug
EUR 416.4
Human Recombinant Transforming Growth Factor-alpha (TGF-alpha), biologically active
TGFA15-R-100
Alpha Diagnostics
100 ug
EUR 634.8
Human Recombinant Transforming Growth Factor-alpha (TGF-alpha), biologically active
TGFA15-R-1000
Alpha Diagnostics
1000 ug
EUR 4225.2
Human Recombinant Transforming Growth Factor-alpha (TGF-alpha), biologically active
TGFA15-R-20
Alpha Diagnostics
20 ug
EUR 270
Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma)-biotinylated
ACTB24-BTN
Alpha Diagnostics
50 ul
EUR 489.6
Comparability of Droplet Digital PCR versus qPCR Measurements on the Worldwide Scale for the Molecular Monitoring of Persistent Myeloid Leukemia Sufferers
Background: BCR-ABL1/ABL1 p210 measurement by quantitative polymerase chain response (qPCR) is used worldwide to watch the molecular response in persistent myeloid leukemia (CML) sufferers. Droplet digital polymerase chain response (ddPCR) appears to indicate a higher sensitivity than qPCR, most likely because of the excessive variety of replicates analyzed in ddPCR for the comparability. Moreover, in a just lately printed comparability, ddPCR measurements weren’t adequately reworked into Worldwide Scale (IS).
Technique: We’ve analyzed 50 CML sufferers and ten non-CML donors in parallel by qPCR and ddPCR. To the very best of our data, that is the primary examine evaluating each methods below related circumstances, with BCR-ABL1/ABL1 measurements carried out through each methods reworked into IS.
Outcomes: Qualitative and quantitative comparisons confirmed wonderful outcomes. The qualitative correlation confirmed a Kappa index of 0.94 (95% confidence interval [CI] 0.90-0.98) (P < 0.001). Within the quantitative comparability, absolutely the intra-class correlation coefficient was 0.868 (95% CI 0.734-0.937; P < 0.001), and Lin’s concordance correlation coefficient was 0.863.
The Passing-Bablock check indicated a slight proportional distinction between qPCR and ddPCR. A quantitative and qualitative subanalysis together with 40 sufferers with a molecular response of three.Zero or deeper confirmed related ends in each check. As well as, the proportional distinction within the Passing-Bablock check disappeared. There have been no variations within the sensitivity for BCR-ABL1 detection between qPCR and ddPCR (McNemar check, P = 0.5).
Conclusions: In conclusion, our outcomes present superb quantitative and qualitative correlations between BCR-ABL1/ABL1 p210 outcomes obtained by qPCR and by ddPCR and make sure earlier scarce information concerning the shortage of a rise in sensitivity of ddPCR over qPCR on this setting.
Anti-Human IgG
DB-173-0.1
DB Biotech
100 μl
EUR 254.4
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-173-0.2
DB Biotech
200 μl
EUR 357.6
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-173-0.5
DB Biotech
500 μl
EUR 460.8
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-173-1
DB Biotech
1 ml
EUR 735.6
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-173-RTU-15
DB Biotech
15 ml
EUR 426
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB-173-RTU-7
DB Biotech
7 ml
EUR 277.2
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB-174-0.1
DB Biotech
100 μl
EUR 254.4
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-174-0.2
DB Biotech
200 μl
EUR 357.6
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-174-0.5
DB Biotech
500 μl
EUR 460.8
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-174-1
DB Biotech
1 ml
EUR 735.6
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated
Anti-Human IgG
DB-174-RTU-15
DB Biotech
15 ml
EUR 426
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB-174-RTU-7
DB Biotech
7 ml
EUR 277.2
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB173RTU-15
DB Biotech
15 ml
EUR 426
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB173RTU-7
DB Biotech
7 ml
EUR 277.2
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB174RTU-15
DB Biotech
15 ml
EUR 426
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
Anti-Human IgG
DB174RTU-7
DB Biotech
7 ml
EUR 277.2
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)
anti-human CCR1
20R-3028
Fitzgerald
200 ug
EUR 704.4
Description: Goat anti-human CCR1 antibody
anti-human RecQL4
AR05-PA0007
Abfrontier
100 ul
EUR 400.8
Description: Rabbit polyclonal to human RecQL4
anti-human Albumin
LF-PA10001
Abfrontier
100 ug
EUR 483.6
Description: Rabbit polyclonal to human Albumin
Human anti AMPH(anti amphiphysin) ELISA Kit
EH2621
FN Test
96T
EUR 628.92
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml
Human anti-TPO(anti-thrombopoietin) ELISA Kit
EH4147
FN Test
96T
EUR 628.92
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens ;Sensitivity: 0.938 ng/ml
Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *
V100125
AAT Bioquest
50 ug
EUR 367.2
Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *
V100126
AAT Bioquest
1 mg
EUR 471.6
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-10x96wellstestplate
Cloud-Clone
10x96-wells test plate
EUR 6777.36
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x48wellstestplate
Cloud-Clone
1x48-wells test plate
EUR 663.31
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x96wellstestplate
Cloud-Clone
1x96-wells test plate
EUR 896.16
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-5x96wellstestplate
Cloud-Clone
5x96-wells test plate
EUR 3672.72
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.
Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
4-AEA465Hu
Cloud-Clone
EUR 6837.60
EUR 3613.20
EUR 896.40
10 plates of 96 wells
5 plates of 96 wells
1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
Anti-human CD47 mAb
CM096-100ug
SAB
100ug
EUR 416.4
Anti-human CD47 mAb
CM096-25ug
SAB
25ug
EUR 242.4
Anti-human CD45 mAb
CM100-100ug
SAB
100ug
EUR 146.4
Anti-human CD45 mAb
CM100-25ug
SAB
25ug
EUR 103.2
Goat Anti-Human IgA
C020242-1mg
Unibiotest
1mg
EUR 272.4
Goat Anti-Human IgA
C020242-50mg
Unibiotest
50mg
EUR 3162
Anti-Human IgG:HRP Conjugate
F163
Cygnus Technologies
12 ml
EUR 585.6
Description: Anti-Human IgG:HRP Conjugate by Cygnus Technologies is available in Europe via Gentaur.
anti- Human IgA antibody
FNab04075
FN Test
100µg
EUR 702
Description: Antibody raised against Human IgA
anti- Human IgA antibody
FNab04076
FN Test
100µg
EUR 702
Description: Antibody raised against Human IgA
anti- Human IgG antibody
FNab04077
FN Test
100µg
EUR 702
Description: Antibody raised against Human IgG
Goat anti Human IgE
70R-IG015
Fitzgerald
1 mg
EUR 418.8
Description: Affinity purified Goat anti Human IgE antibody
Goat anti Human IgG
70C-CR6047GAP
Fitzgerald
1 mg
EUR 178.8
Description: Affinity purified Goat anti Human IgG antibody
Goat anti Human IgM
70-B9022GA00-A0
Fitzgerald
5 mg
EUR 230.4
Description: Goat anti Human IgM antibody
Goat anti Human IgG
41C-CB0957
Fitzgerald
5 mg
EUR 415.2
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41C-CB0972
Fitzgerald
2 mg
EUR 316.8
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgM
41C-CB0993
Fitzgerald
1 mg
EUR 244.8
Description: Goat anti Human IgM secondary antibody
Goat anti Human IgG
41R-1026
Fitzgerald
2 mg
EUR 164.4
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1027
Fitzgerald
2 mg
EUR 223.2
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1028
Fitzgerald
1 mg
EUR 218.4
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1029
Fitzgerald
2 mg
EUR 223.2
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1030
Fitzgerald
1 mg
EUR 165.6
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1031
Fitzgerald
1 mg
EUR 223.2
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1032
Fitzgerald
1 mg
EUR 268.8
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1033
Fitzgerald
1 mg
EUR 295.2
Description: Goat anti Human IgG secondary antibody
Goat anti Human IgG
41R-1034
Fitzgerald
1 mg
EUR 223.2
Description: Goat anti Human IgG secondary antibody
Mouse anti Human IgM
41R-1586
Fitzgerald
100 ug
EUR 318
Description: Monoclonal Mouse anti Human IgM antibody
Goat anti Human IgE
40-IG10
Fitzgerald
1 ml
EUR 159.6
Description: Goat anti Human IgE antibody
Sheep anti Human IgE
40-IG11
Fitzgerald
1 ml
EUR 159.6
Description: Sheep anti Human IgE antibody
Rabbit anti Human IgG
40C-CB0943
Fitzgerald
1 mg
EUR 298.8
Description: Rabbit anti Human IgG secondary antibody
Rabbit anti Human IgG
40C-CB0950
Fitzgerald
5 mg
EUR 457.2
Description: Rabbit anti Human IgG secondary antibody
Rabbit anti Human IgG
40C-CB0971
Fitzgerald
2 mg
EUR 386.4
Description: Rabbit anti Human IgG secondary antibody
Rabbit anti Human IgM
40C-CB9100
Fitzgerald
2 mg
EUR 339.6
Description: Rabbit anti Human IgM secondary antibody
Rabbit anti Human IgA
40C-CB9114
Fitzgerald
2 mg
EUR 310.8
Description: Rabbit anti Human IgA secondary antibody
Sheep anti Human IgM
40C-CR2023S
Fitzgerald
1 ml
EUR 289.2
Description: Sheep anti Human IgM antibody
Mouse anti Human IgE
40R-1000
Fitzgerald
100 ug
EUR 318
Description: Mouse anti Human IgE antibody
Mouse anti Human IgA
40R-1001
Fitzgerald
100 ug
EUR 318
Description: Mouse anti Human IgA antibody
Mouse anti Human IgG
40R-1003
Fitzgerald
100 ug
EUR 318
Description: Mouse anti Human IgG antibody
Mouse anti Human IgE
40R-1004
Fitzgerald
100 ug
EUR 318
Description: Mouse anti Human IgE antibody
Mouse anti Human IgM
40R-1005
Fitzgerald
100 ug
EUR 318
Description: Mouse anti Human IgM antibody
Mouse anti Human IgA
40R-1006
Fitzgerald
100 ug
EUR 318
Description: Mouse anti Human IgA antibody
Rat anti Human IgG1
40R-1007
Fitzgerald
100 ug
EUR 318
Description: Rat monoclonal Rat anti Human IgG1 antibody
Mouse anti Human IgA
40R-1011
Fitzgerald
500 ug
EUR 678
Description: Mouse anti Human IgA secondary antibody
Mouse anti Human IgA
40R-1012
Fitzgerald
500 ug
EUR 678
Description: Mouse anti Human IgA secondary antibody
Mouse anti Human IgG3
40R-1015
Fitzgerald
1 mg
EUR 496.8
Description: Mouse anti Human IgG3 antibody
Rat anti Human IgG
40R-1588
Fitzgerald
100 ug
EUR 318
Description: Rat monoclonal Rat anti Human IgG antibody
Goat anti Human IgG
41-XG56
Fitzgerald
1 mg
EUR 121.2
Description: Goat anti Human IgG secondary antibody
Sheep anti Human IgA
20-1266
Fitzgerald
1ml
EUR 776.4
Description: Sheep polyclonal Human IgA secondary antibody
Sheep anti Human IgG
20-1377
Fitzgerald
1ml
EUR 5667.6
Description: Sheep polyclonal Human IgG secondary antibody
Goat anti Human IgA
20-B9001G000-S0
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgA antibody
Goat anti Human IgM
20-B9022G000-S2
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgM antibody
Rabbit anti Human IgE
20-IR77
Fitzgerald
1 ml
EUR 230.4
Description: Rabbit anti Human IgE antibody
Goat anti Human IgA
20-S11030GND1-D
Fitzgerald
5 mg
EUR 230.4
Description: Goat anti Human IgA antibody
Goat anti Human IgA
20-S1103GND1-D0
Fitzgerald
10 ml
EUR 230.4
Description: Goat anti Human IgA antibody
Goat anti Human IgA
20-S1111G000-S4
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgA antibody
Goat anti Human IgA
20-S1111G000-V0
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgA antibody
Goat anti Human IgA
20-S1111GOOO-S4
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgA antibody
Goat anti Human IgM
20-S1303GND1-D0
Fitzgerald
10 ml
EUR 230.4
Description: Goat anti Human IgM antibody
Goat anti Human IgM
20-S1304GA01
Fitzgerald
5 ml
EUR 165.6
Description: Goat anti Human IgM antibody
Goat anti Human IgM
20-S1311G000-S4
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgM antibody
Goat anti Human IgM
20-S1311G000-V0
Fitzgerald
10 ml
EUR 133.2
Description: Goat anti Human IgM antibody
Goat anti Human IgM
20-S1311G001-S4
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgM antibody
Goat anti Human IgE
20-S2001G000-S4
Fitzgerald
10 ml
EUR 152.4
Description: Goat anti Human IgE antibody
Goat anti Human IgE
20-S2001G000-V0
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgE antibody
Goat anti Human IgE
20-S2001G000-VO
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgE antibody
Goat anti Human IgE
20-S2003GND1-D0
Fitzgerald
10 ml
EUR 230.4
Description: Goat anti Human IgE antibody
Goat anti Human IgM
20-S4113GN00-D4
Fitzgerald
10 ml
EUR 230.4
Description: Goat anti Human IgM antibody
Goat anti Human IgM
20-S5170GND1-D0
Fitzgerald
10 ml
EUR 230.4
Description: Goat anti Human IgM antibody
Goat anti Human IgA
20-S5491G000-S4
Fitzgerald
10 ml
EUR 159.6
Description: Goat anti Human IgA antibody
Sheep anti Human IgA
20C-CR6043SP
Fitzgerald
1 ml
EUR 265.2
Description: Sheep anti Human IgA antibody
Sheep anti Human IgA
20C-CR6043SP-S
Fitzgerald
1 ml
EUR 265.2
Description: Sheep anti Human IgA antibody
Mouse anti Human IgM
10C-CR2023M2
Fitzgerald
1 mg
EUR 184.8
Description: Mouse anti Human IgM antibody
Mouse anti Human IgM
10C-CR2023M3
Fitzgerald
1 mg
EUR 184.8
Description: Mouse anti Human IgM antibody
Mouse anti Human IgA
10C-CR6043M1
Fitzgerald
1 mg
EUR 289.2
Description: Mouse anti Human IgA antibody
Mouse anti Human IgE
10C-CR6046M3
Fitzgerald
1 mg
EUR 198
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10C-CR6046M4
Fitzgerald
1 mg
EUR 198
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10C-CR6046M5
Fitzgerald
1 mg
EUR 198
Description: Mouse anti Human IgE antibody
Mouse anti Human IgG
10C-CR6047M1
Fitzgerald
1 mg
EUR 211.2
Description: Mouse anti Human IgG antibody
Mouse anti Human IgG
10C-CR6047M2
Fitzgerald
1 mg
EUR 211.2
Description: Mouse anti Human IgG antibody
Mouse anti Human IgE
10R-I104d
Fitzgerald
1 mg
EUR 536.4
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10R-I104e
Fitzgerald
1 mg
EUR 586.8
Description: Mouse anti Human IgE antibody
Mouse anti Human IgG1
10R-I110a
Fitzgerald
1 ml
EUR 664.8
Description: Mouse anti Human IgG1 antibody
Mouse anti Human IgG1
10R-I127a
Fitzgerald
1 mg
EUR 586.8
Description: Mouse anti Human IgG1 antibody
Mouse anti Human IgG2
10R-I128a
Fitzgerald
1 mg
EUR 586.8
Description: Mouse anti Human IgG2 antibody
Mouse anti Human IgG4
10R-I129a
Fitzgerald
1 mg
EUR 573.6
Description: Mouse anti Human IgG4 antibody
Mouse anti Human IgM
10R-I130a
Fitzgerald
1 mg
EUR 382.8
Description: Mouse anti Human IgM antibody
Mouse anti Human IgM
10R-I130B
Fitzgerald
1 mg
EUR 360
Description: Mouse anti Human IgM antibody
Mouse anti Human IgE
10-1025
Fitzgerald
1 mg
EUR 392.4
Description: Mouse monoclonal Mouse anti Human IgE antibody
Mouse anti Human IgE
10-1026
Fitzgerald
1 mg
EUR 392.4
Description: Mouse monoclonal Mouse anti Human IgE antibody
Mouse anti Human IgE
10-1027
Fitzgerald
1 mg
EUR 392.4
Description: Mouse monoclonal Mouse anti Human IgE antibody
Mouse anti Human IgA
10-7808
Fitzgerald
1 mg
EUR 403.2
Description: Mouse anti Human IgA antibody
Mouse anti Human IgA
10-I05A
Fitzgerald
1 mg
EUR 369.6
Description: Mouse anti Human IgA antibody
Mouse anti Human IgE
10-I104B
Fitzgerald
1 mg
EUR 403.2
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10-I104CC
Fitzgerald
1 mg
EUR 403.2
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10-I10A
Fitzgerald
1 mg
EUR 217.2
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10-I10C
Fitzgerald
1 mg
EUR 217.2
Description: Mouse anti Human IgE antibody
Mouse anti Human IgE
10-I10E
Fitzgerald
1 mg
EUR 594
Description: Mouse anti Human IgE antibody
Mouse anti Human IgM
10-I20A
Fitzgerald
1 mg
EUR 229.2
Description: Mouse anti Human IgM antibody
Mouse anti Human IgM
10-I20B
Fitzgerald
1 mg
EUR 354
Description: Mouse anti Human IgM antibody
Mouse anti Human IgM
10-I20G
Fitzgerald
1 mg
EUR 354
Description: Mouse anti Human IgM antibody
Mouse anti Human IgM
10-I20H
Fitzgerald
1 mg
EUR 224.4
Description: Mouse anti Human IgM antibody
Mouse anti Human IgG1
10-I21A
Fitzgerald
200 ug
EUR 264
Description: Mouse anti Human IgG1 antibody
Mouse anti Human IgG2
10-I22A
Fitzgerald
200 ug
EUR 470.4
Description: Mouse anti Human IgG2 antibody
Mouse anti Human IgG3
10-I23A
Fitzgerald
200 ug
EUR 762
Description: Mouse anti Human IgG3 antibody
Mouse anti Human IgG4
10-I24A
Fitzgerald
200 ug
EUR 470.4
Description: Mouse anti Human IgG4 antibody
Mouse anti Human IgE
10-S9601MND1-M0
Fitzgerald
1 mg
EUR 262.8
Description: Mouse anti Human IgE antibody
Anti-CCR10 (human) Antibody
A04731
BosterBio
200ug
EUR 476.4
Description: Goat Polyclonal CCR10 (human) Antibody. Validated in ELISA, IF, IHC and tested in Human.
Anti-TRAIL (human) Antibody
A00466-2
BosterBio
100ul
EUR 500.4
Description: Rabbit Polyclonal TRAIL (human) Antibody. Validated in IF, IHC and tested in Human.
Anti-CCR4 (human) Antibody
A00755-1
BosterBio
200ug
EUR 476.4
Description: Goat Polyclonal CCR4 (human) Antibody. Validated in IF, IHC and tested in Human.
Anti-Human IBA1 Antibody
M01394-2
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human.
Anti-Human IBA1 Antibody
M01394-3
BosterBio
100ul
EUR 476.4
Description: Chicken Polyclonal Human IBA1 Antibody. Validated in IF, IHC, WB and tested in Human.
anti-Human IgG (3E8)
LF-MA0163
Abfrontier
100 ul
EUR 400.8
Description: Mouse monoclonal to Human IgG
anti-Human IgM (13A11)
LF-MA0164
Abfrontier
100 ul
EUR 400.8
Description: Mouse monoclonal to Human IgM
anti-Human IgA (47C12)
LF-MA0165
Abfrontier
100 ul
EUR 400.8
Description: Mouse monoclonal to Human IgA
anti-Human CD9 (1B6)
LF-MA0375
Abfrontier
100 ul
EUR 400.8
Description: Mouse monoclonal to CD9
anti-Human PGIS (3C8)
LF-MA0376
Abfrontier
100 ul
EUR 400.8
Description: Mouse monoclonal to PGIS
anti-Human P16 (5A8A4)
LF-MA30115
Abfrontier
100 ul
EUR 583.2
Description: Mouse Monoclonal to Human P16
anti-human Albumin (2H3D1)
LF-MA30221
Abfrontier
100 ul
EUR 583.2
Description: Mouse Monoclonal to human Albumin