PCR versus qPCR Measurements on the Worldwide Scale for the Molecular Monitoring of Persistent Myeloid Leukemia

A Simplified Quantitative Actual-Time PCR Assay for Monitoring SARS-CoV-2 Development in Cell Tradition

Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has contaminated hundreds of thousands inside just some months, inflicting extreme respiratory illness and mortality. Assays to watch SARS-CoV-2 development in vitro rely on time-consuming and dear RNA extraction steps, hampering progress in primary analysis and drug growth efforts. Right here, we developed a simplified quantitative real-time PCR assay that bypasses viral RNA extraction steps and might monitor SARS-CoV-2 development from a small quantity of cell tradition supernatants. As well as, we present that this method is definitely adaptable to quite a few different RNA and DNA viruses.

Utilizing this assay, we screened the actions of a lot of compounds that had been predicted to change SARS-CoV-2 entry and replication in addition to HIV-1-specific medication in a proof-of-concept examine. We discovered that E64D (inhibitor of endosomal proteases cathepsin B and L) and apilimod (endosomal trafficking inhibitor) potently decreased the quantity of SARS-CoV-2 RNA in cell tradition supernatants with minimal cytotoxicity.

Surprisingly, we discovered that the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) equally decreased SARS-CoV-2 RNA ranges in supernatants, suggesting that entry may also be mediated by an alternate pathway. HIV-1-specific inhibitors nevirapine (a nonnucleoside reverse transcriptase inhibitor [NNRTI]), amprenavir (a protease inhibitor), and allosteric integrase inhibitor 2 (ALLINI-2) modestly inhibited SARS-CoV-2 replication, albeit the 50% inhibitory focus (IC₅₀) values had been a lot increased than that required for HIV-1.

Taking the info collectively, this simplified assay will expedite primary SARS-CoV-2 analysis, be amenable to mid-throughput screening assays (i.e., drug, CRISPR, small interfering RNA [siRNA], and so forth.), and be relevant to a broad variety of RNA and DNA viruses.

IMPORTANCE Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of the coronavirus illness 2019 (COVID-19) pandemic, is constant to trigger immense respiratory illness and social and financial disruptions.

Standard assays that monitor SARS-CoV-2 development in cell tradition depend on pricey and time-consuming RNA extraction procedures, hampering progress in primary SARS-CoV-2 analysis and growth of efficient therapeutics. Right here, we developed a easy quantitative real-time PCR assay to monitor SARS-CoV-2 development in cell tradition supernatants that doesn’t necessitate RNA extraction and that’s as correct and delicate as present strategies. In a proof-of-concept display screen, we discovered that E64D, apilimod, EIPA, and remdesivir can considerably impede SARS-Cov-2 replication, offering novel perception into viral entry and replication mechanisms.

As well as, we present that this method is definitely adaptable to quite a few different RNA and DNA viruses. This simplified assay will undoubtedly expedite primary SARS-CoV-2 and virology analysis and be amenable to make use of in drug screening platforms to establish therapeutics in opposition to SARS-CoV-2.

Rabbit Anti-Human alpha-Tubulin IgG
TUBA13-A	Alpha Diagnostics	100 ul	EUR 482.00

Rabbit Anti-Human Fodrin-alpha (spectrin-alpha) IgG, aff pure
FOD11-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human Estrogen receptor alpha (ER-alpha) peptide IgG
ERA12-A	Alpha Diagnostics	100 ug	EUR 482.00

Chicken Anti-Human alpha 1 anti-trypsin IgG/Y
A1AT12-A	Alpha Diagnostics	100 ul	EUR 482.00

Polyclonal Goat anti-GST α-form
GST-ANTI-1	Detroit R&D	50 uL	EUR 280.00

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280.00

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280.00

Rabbit Anti-Human p73 alpha antiserum # 2
P73A12-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Human Synuclein-alpha antiserum # 1
SYN11-S	Alpha Diagnostics	100 ul	EUR 457.00

Goat Anti-human Chorionic Gonadotropin Alpha (hCG-Alpha) IgG aff pure
HCGA13-A	Alpha Diagnostics	1 mg	EUR 286.00

Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure
FSHA12-M	Alpha Diagnostics	1 mg	EUR 482.00

Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure
LHA13-A	Alpha Diagnostics	1 mg	EUR 286.00

Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure
TOP2A11-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Mouse Neprilysin-alpha (NEPL-alpha) antiserum
NEPLA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Monoclonal Anti-Human Chorionic Gonadotropin Alpha (hCG-alpha) IgG #1, aff pure
HCGA11-M	Alpha Diagnostics	1 mg	EUR 286.00

Monoclonal Anti-human Chronionic Gonadotropin Alpha (hCG-Alpha) IgG #2, caff pure
HCGA12-M	Alpha Diagnostics	1 mg	EUR 286.00

Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #2
RELMA12-S	Alpha Diagnostics	100 ul	EUR 457.00

Goat Anti-human Alpha fetoprotein (AFP) IgG, pure
AFP13-A	Alpha Diagnostics	1 mg	EUR 651.00

Rabbit Anti-Human p73 alpha/beta antiserum # 1
P73A11-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Human alpha-Tubulin IgG-Biotin Conjugate
TUBA13-BTN	Alpha Diagnostics	100 ul	EUR 469.00

Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate
TUBA13-HRP	Alpha Diagnostics	100 ul	EUR 432.00

Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum
GSTA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Monoclonal anti-human Lutenizing Hormone Alpha (LH-Alpha) IgG, clone 2 aff pure
LHA12-M	Alpha Diagnostics	1 mg	EUR 286.00

Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #1 aff. Pure
TNFA11-M	Alpha Diagnostics	100 ug	EUR 482.00

Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure
TNFA12-M	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Mouse Neprilysin-alpha (NEPL-alpha) IgG (aff pure)
NEPLA11-A	Alpha Diagnostics	100 ug	EUR 482.00

Goat Anti-Ferret IgA (alpha), unlabeled
70519	Alpha Diagnostics	0.5 mg	EUR 213.00

Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 1)
AFP11-M	Alpha Diagnostics	1 mg	EUR 482.00

Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 2)
AFP12-M	Alpha Diagnostics	1 mg	EUR 482.00

Rabbit Anti-Human HE2 alpha IgG #1, aff pure
HE2A11-A	Alpha Diagnostics	100 ug	EUR 482.00

Chicken Anti-Human Alpha 2 Macroglobulin (A2M) IgG/Y
A2MG13-A	Alpha Diagnostics	100 ul	EUR 482.00

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, unlabeled
A1AT11-A	Alpha Diagnostics	100 ul	EUR 408.00

Goat Anti-Human IgA (alpha chain sp.), aff pure
10019	Alpha Diagnostics	0.5 mg	EUR 202.00

Rabbit Anti-Human p73 alpha/ IgG # 2, aff pure
P73A12-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human Synuclein-alpha IgG # 1, aff pure
SYN11-A	Alpha Diagnostics	100 ug	EUR 482.00

Monoclonal anti-human Follicle Stimulating Hormone Alpha (FSH-Alpha), IgG clone 2 aff pure
FSHA11-M	Alpha Diagnostics	1 mg	EUR 482.00

Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) IgG #2, aff. Pure
RELMA12-A	Alpha Diagnostics	100 ug	EUR 482.00

Goat Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure (neutralzing)
TNFA13-A	Alpha Diagnostics	100 ul	EUR 482.00

Goat Anti-Follicle Stimulating Hormone Alpha (FSH-Alpha) IgG aff pure
FSH13-A	Alpha Diagnostics	1 mg	EUR 482.00

monoclonal Anti-Rat Resistin-Like protein alpha (RELM-alpha/FIZZ1) ascites
RELMA14-M	Alpha Diagnostics	100 ul	EUR 482.00

Rabbit Anti-Human Core-binding factor alpha (CBFA1) antiserum #1
CBFA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Sheep Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure
ACT11-A	Alpha Diagnostics	100 ug	EUR 408.00

Monoclonal Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure
ACT12-M	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human Alpha Defensin 4 (NP-4) antiserum #1
HDEFA41-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Human Alpha Defensin 5 (NP-5),antiserum #1
HDEFA51-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Human Alpha Defensin 6 (NP-6) antiserum #1
HDEFA61-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) Antiserum #1
GAA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, unlabeled
A2MG11-A	Alpha Diagnostics	100 ul	EUR 347.00

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, Biotin conjugate
A1AT11-BT	Alpha Diagnostics	100 ul	EUR 408.00

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, HRP conjugate
A1AT11-HRP	Alpha Diagnostics	100 ul	EUR 408.00

Goat Anti-Human IgA (alpha chain sp.) IgG-Biotin Conjugate
10040	Alpha Diagnostics	0.5 mg	EUR 202.00

Goat Anti-Human IgA (alpha chain sp.)-Alk. Phosphatase conjugate
10050	Alpha Diagnostics	0.5 ml	EUR 225.00

Human Anti-Alpha Fodrin IgG ELISA kit, 96 tests, Quantitative
3300-160-AFG	Alpha Diagnostics	1 kit	EUR 590.00

Rabbit Anti-Human p73 alpha/beta IgG # 1, aff pure
P73A11-A	Alpha Diagnostics	100 ug	EUR 482.00

Mouse Monoclonal Anti-Human Synuclein-alpha protein IgG, aff pure
SYNRP-84816-M	Alpha Diagnostics	100 ug	EUR 482.00

Goat Anti-Ferret IgA (alpha)-HRP conjugate
70520	Alpha Diagnostics	0.5 ml	EUR 238.00

Rabbit Anti-C. perfringens alpha toxin antiserum
CPA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Rat Synuclein-alpha antiserum # 2
SYN12-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #1
RELMA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Rat monoclonal Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) ascites
RELMA13-M	Alpha Diagnostics	100 ul	EUR 482.00

Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha
RP-675	Alpha Diagnostics	1 ug	EUR 286.00

Rabbit Anti-Human IL-2 receptor alpha, soluble (IL2RsA/CD25) IgG
CD251-A	Alpha Diagnostics	100 ul	EUR 482.00

Monoclonal Anti-Human/mouse/rat HSP90 alpha/beta IgG, aff pure
HSP901-M	Alpha Diagnostics	100 ul	EUR 482.00

Rabbit Anti-Human GABAa Receptor alpha 2 (GAA2) IgG, aff. Pure
GAA21-A	Alpha Diagnostics	100 ug	EUR 482.00

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, Biotin conjugate
A2MG11-BT	Alpha Diagnostics	100 ul	EUR 286.00

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, HRP conjugate
A2MG11-HRP	Alpha Diagnostics	100 ul	EUR 286.00

Chicken Anti-Human Alpha 1 acid glycoprotein (A1AG) IgG/Y, unlabeled
A1AG12-A	Alpha Diagnostics	100 ul	EUR 482.00

Monoclonal Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein, ascites
A1AG12-M	Alpha Diagnostics	100 ul	EUR 482.00

Monoclonal Anti-Human Lutenizing Hormone (LH), alpha chain IgG, aff pure
LHA14-M	Alpha Diagnostics	1 mg	EUR 286.00

Goat Anti-Rat IgA (alpha chain)-HRP conjugate
50020	Alpha Diagnostics	0.5 ml	EUR 225.00

Goat Anti-Rat IgA (alpha chain)-Biotin conjugate
50040	Alpha Diagnostics	0.5 ml	EUR 225.00

Rabbit Anti-Goat IgA (alpha chain)-HRP conjugate
30120	Alpha Diagnostics	0.5 ml	EUR 213.00

Rabbit Anti-Goat IgA (alpha chain)-FITC conjugate
30130	Alpha Diagnostics	0.5 ml	EUR 213.00

Goat Anti-Rabbit IgA (alpha chain)-HRP conjugate
20029	Alpha Diagnostics	0.5 ml	EUR 263.00

Goat Anti-Chicken IgA (alpha chain)-HRP conjugate
60220	Alpha Diagnostics	0.5 ml	EUR 213.00

Goat Anti-Chicken IgA (alpha chain)-FITC Conjugate
60230	Alpha Diagnostics	0.5 ml	EUR 213.00

Goat Anti-Chicken IgA (alpha chain)-biotin conjugate
60240	Alpha Diagnostics	0.5 ml	EUR 225.00

Goat Anti-Monkey IgA (alpha-chain specific), unlabeled
70043	Alpha Diagnostics	0.5 mg	EUR 225.00

Monoclonal Anti-Bovine S100 alpha ab # 2, ascites
S1005-M	Alpha Diagnostics	100 ul	EUR 482.00

Human Fodrin-alpha (spectrin-alpha) control/blocking peptide
FOD11-P	Alpha Diagnostics	100 ug	EUR 164.00

Recombinant Human Alpha-Synuclein
RP-847	Alpha Diagnostics	20 ug	EUR 164.00

Rabbit Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) IgG#1, aff. pure
RELMA11-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human/mouse/rat HSP90 alpha (hsp90AB1) peptide IgG, aff pure
HSP903-A	Alpha Diagnostics	100 ul	EUR 482.00

Rabbit Anti-Human Alpha Defensin 4 (NP-4), IgG #1, aff pure
HDEFA41-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human Alpha Defensin 5 (NP-5),, IgG #1, aff pure
HDEFA51-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human Alpha Defensin 6 (NP-6) IgG #1, aff pure
HDEFA61-A	Alpha Diagnostics	100 ug	EUR 482.00

Monoclonal Anti-Human Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG
FETA13-M	Alpha Diagnostics	100 ug	EUR 482.00

Sheep Anti-Human Serum Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG
FETA15-A	Alpha Diagnostics	100 ul	EUR 408.00

Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) IgG #1, aff pure
GAA11-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human/Rat GABAa Receptor alpha 3 (GAA3) IgG, aff pure
GAA31-A	Alpha Diagnostics	100 ug	EUR 482.00

Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, unlabeled
A1AG11-A	Alpha Diagnostics	100 ul	EUR 408.00

Rabbit Anti-Human interleukin 13 receptor, alpha 2 (IL13RA2) IgG (aff pure)
AB-23040-A	Alpha Diagnostics	100ug	EUR 482.00

Rabbit Anti-Human PUMA-alpha (p53-upregulated modulator of apoptosis) antiserum #1
PUMA11-S	Alpha Diagnostics	100 ul	EUR 457.00

Rabbit Anti-Human PUMA-alpha (p53-upregulated modulator of apoptosis) antiserum #1
PUMA12-S	Alpha Diagnostics	100 ul	EUR 457.00

Mouse Monoclonal Anti-Human Luteinizing Hormone (LH), alpha chain IgG, aff pure
LH13-M	Alpha Diagnostics	1 mg	EUR 286.00

Monoclonal Anti-human Lutenizing Hormone (LH- Alpha) IgG, clone 1 aff pure
LHA11-M	Alpha Diagnostics	1 mg	EUR 286.00

human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide
TOP2A11-P	Alpha Diagnostics	100 ug	EUR 164.00

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma)-biotinylated
ACTB24-BTN	Alpha Diagnostics	50 ul	EUR 408.00

Comparability of Droplet Digital PCR versus qPCR Measurements on the Worldwide Scale for the Molecular Monitoring of Persistent Myeloid Leukemia Sufferers

Background: BCR-ABL1/ABL1 p210 measurement by quantitative polymerase chain response (qPCR) is used worldwide to watch the molecular response in persistent myeloid leukemia (CML) sufferers. Droplet digital polymerase chain response (ddPCR) appears to indicate a higher sensitivity than qPCR, most likely because of the excessive variety of replicates analyzed in ddPCR for the comparability. Moreover, in a just lately printed comparability, ddPCR measurements weren’t adequately reworked into Worldwide Scale (IS).

Technique: We’ve analyzed 50 CML sufferers and ten non-CML donors in parallel by qPCR and ddPCR. To the very best of our data, that is the primary examine evaluating each methods below related circumstances, with BCR-ABL1/ABL1 measurements carried out through each methods reworked into IS.

Outcomes: Qualitative and quantitative comparisons confirmed wonderful outcomes. The qualitative correlation confirmed a Kappa index of 0.94 (95% confidence interval [CI] 0.90-0.98) (P < 0.001). Within the quantitative comparability, absolutely the intra-class correlation coefficient was 0.868 (95% CI 0.734-0.937; P < 0.001), and Lin’s concordance correlation coefficient was 0.863.

The Passing-Bablock check indicated a slight proportional distinction between qPCR and ddPCR. A quantitative and qualitative subanalysis together with 40 sufferers with a molecular response of three.Zero or deeper confirmed related ends in each check. As well as, the proportional distinction within the Passing-Bablock check disappeared. There have been no variations within the sensitivity for BCR-ABL1 detection between qPCR and ddPCR (McNemar check, P = 0.5).

Conclusions: In conclusion, our outcomes present superb quantitative and qualitative correlations between BCR-ABL1/ABL1 p210 outcomes obtained by qPCR and by ddPCR and make sure earlier scarce information concerning the shortage of a rise in sensitivity of ddPCR over qPCR on this setting.

Polyclonal Goat anti-GST μ-form
GST-ANTI-2	Detroit R&D	50 uL	EUR 280.00

Polyclonal Goat anti-GST p-form
GST-ANTI-3	Detroit R&D	50 uL	EUR 280.00

anti-human RecQL4
AR05-PA0007	Abfrontier	100 ul	EUR 334.00
Description: Rabbit polyclonal to human RecQL4

anti-human CCR1
20R-3028	Fitzgerald	200 ug	EUR 587.00
Description: Goat anti-human CCR1 antibody

Anti-Human IgG
DB-173-0.1	DB Biotech	100 μl	EUR 212.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-173-0.2	DB Biotech	200 μl	EUR 298.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-173-0.5	DB Biotech	500 μl	EUR 384.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-173-1	DB Biotech	1 ml	EUR 613.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-173-RTU-15	DB Biotech	15 ml	EUR 355.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB-173-RTU-7	DB Biotech	7 ml	EUR 231.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB-174-0.1	DB Biotech	100 μl	EUR 212.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-174-0.2	DB Biotech	200 μl	EUR 298.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-174-0.5	DB Biotech	500 μl	EUR 384.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-174-1	DB Biotech	1 ml	EUR 613.00
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG
DB-174-RTU-15	DB Biotech	15 ml	EUR 355.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB-174-RTU-7	DB Biotech	7 ml	EUR 231.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB173RTU-15	DB Biotech	15 ml	EUR 355.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB173RTU-7	DB Biotech	7 ml	EUR 231.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB174RTU-15	DB Biotech	15 ml	EUR 355.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG
DB174RTU-7	DB Biotech	7 ml	EUR 231.00
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

anti-human Albumin
LF-PA10001	Abfrontier	100 ug	EUR 403.00
Description: Rabbit polyclonal to human Albumin

Goat anti-Human anti-thrombin polyclonal antibody
CABT-L487	Creative Diagnostics	500ug	EUR 715.00

Sheep anti-Human anti-thrombin polyclonal antibody
CABT-L488	Creative Diagnostics	500ug	EUR 663.00

Sheep anti-Human anti-thrombin polyclonal antibody
CABT-L489	Creative Diagnostics	100ug	EUR 663.00

Sheep anti-Human anti-thrombin polyclonal antibody
CABT-L490	Creative Diagnostics	100ug	EUR 663.00

Sheep anti-Human anti-thrombin polyclonal antibody
CABT-L491	Creative Diagnostics	100ug	EUR 663.00

Human anti-TPO(anti-thrombopoietin) ELISA Kit
EH4147	FN Test	96T	EUR 524.10
Detection range: 1.563-100 ng/ml Alias: anti-TPO
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens ;Sensitivity: 0.938 ng/ml

Human anti AMPH(anti amphiphysin) ELISA Kit
EH2621	FN Test	96T	EUR 524.10
Detection range: 31.25-2000 pg/ml Uniprot ID: P49418 Alias: anti-AMPH
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *
V100125	AAT Bioquest	50 ug	EUR 306.00
R-phrase: H-Phrase: Symbol for dangerous compounds: UNSPEC Code:

Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *
V100126	AAT Bioquest	1 mg	EUR 393.00
R-phrase: H-Phrase: Symbol for dangerous compounds: UNSPEC Code:

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5647.80
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 552.76
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 746.80
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
AEA465Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 3060.60
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate CV(%) = SD/meanX100 Intra-Assay: CV<10% Int Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit
4-AEA465Hu	Cloud-Clone	EUR 5698.00 EUR 3011.00 EUR 747.00	0 1 2
Known also as Anti-Sperm Antibody elisa. Alternative names of the recognized antigen: Anti-Spermatozoa Antibodies Sperm Antibodies Antisperm Antibodies
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)
ELK8071	ELK Biotech	1 plate of 96 wells	EUR 432.00
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate soluti Show more
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Goat Anti-Human IgA
C020242-1mg	Unibiotest	1mg	EUR 227.00

Goat Anti-Human IgA
C020242-50mg	Unibiotest	50mg	EUR 2635.00

Rabbit Anti Human IgG
E61I00101	EnoGene	100ug	EUR 343.00

Goat Anti Human IgG
E61I00201	EnoGene	1mg	EUR 274.00

Goat Anti Human IgM
E61I00401	EnoGene	100ug	EUR 343.00

Mouse Anti Human IgM
E61I00402	EnoGene	100ug	EUR 343.00

Goat anti human IgA
E61I01201	EnoGene	1mg	EUR 611.00

Mouse anti human IgA
E61I02101	EnoGene	100ug	EUR 343.00

Goat anti human IgA
E61I02102	EnoGene	100ug	EUR 343.00

Mouse anti human IgE
E61I02201	EnoGene	100ug	EUR 343.00

Anti-Human IgG:HRP Conjugate
F163	Cygnus Technologies	12 ml	EUR 488.00
Product category: Antibodies
Description: Anti-Human IgG:HRP Conjugate by Cygnus Technologies is available in Europe via Gentaur.

Goat anti- human IgA
FNab09895	FN Test	100µg	EUR 548.75
Recommended dilution: WB: 1:1000-1:10000 Immunogen: human IgA Research Area: Immunology
Description: Antibody raised against Goat human IgA

Goat anti- human IgG
FNab09896	FN Test	100µg	Ask for price
Recommended dilution: WB: 1:1000-1:10000 Immunogen: human IgG Research Area: Immunology
Description: Antibody raised against Goat human IgG

Goat anti- human IgM
FNab09908	FN Test	100µg	EUR 548.75
Recommended dilution: WB: 1:1000-1:10000 Immunogen: human IgM Research Area: Immunology
Description: Antibody raised against Goat human IgM

anti- Human IgA antibody
FNab04075	FN Test	100µg	EUR 585.00
Immunogen: immunoglobulin heavy constant alpha 1 Research Area: Immunology, Signal Transduction, cancer, stem cells, Metabolism
Description: Antibody raised against Human IgA

anti- Human IgA antibody
FNab04076	FN Test	100µg	EUR 585.00
Recommended dilution: WB: 1:5000-1:50000 IHC: 1:20000-1:100000 Immunogen: immunoglobulin heavy constant alpha 1 Research Area: Immunology, Signal Transduction, cancer, stem cells, Metabolism
Description: Antibody raised against Human IgA

anti- Human IgG antibody
FNab04077	FN Test	100µg	EUR 585.00
Recommended dilution: WB: 1:2000-1:20000 Immunogen: Human IgG Research Area: Immunology, Signal Transduction, cancer, stem cells, Metabolism
Description: Antibody raised against Human IgG

Goat Anti-Human Albumin
GAHA85-0100	Equitech	100ml	EUR 336.70
Goat Anti-Human Albumin is indicated as RUO. Do not use on humans.

Goat Anti-Human Albumin
GAHA85-0500	Equitech	500ml	EUR 643.50
Goat Anti-Human Albumin is indicated as RUO. Do not use on humans.

Goat, anti-human IgA
IM140	Cygnus Technologies	1 ml	EUR 359.00
Product category: Antibodies
Description: Goat, anti-human IgA by Cygnus Technologies is available in Europe via Gentaur.

Goat, anti-human IgD
IM141	Cygnus Technologies	1 ml	EUR 359.00
Product category: Antibodies
Description: Goat, anti-human IgD by Cygnus Technologies is available in Europe via Gentaur.

Goat, anti-human IgE
IM142	Cygnus Technologies	1 ml	EUR 359.00
Product category: Antibodies
Description: Goat, anti-human IgE by Cygnus Technologies is available in Europe via Gentaur.

Goat, anti-human IgM
IM144	Cygnus Technologies	1 ml	EUR 359.00
Product category: Antibodies
Description: Goat, anti-human IgM by Cygnus Technologies is available in Europe via Gentaur.

Anti-Human IgG1:HRP
IM151	Cygnus Technologies	1 ml	EUR 322.00
Product category: Antibodies
Description: Anti-Human IgG1:HRP by Cygnus Technologies is available in Europe via Gentaur.

Anti-Human IgG2:HRP
IM152	Cygnus Technologies	1 ml	EUR 322.00
Product category: Antibodies
Description: Anti-Human IgG2:HRP by Cygnus Technologies is available in Europe via Gentaur.

Anti-Human IgG3:HRP
IM153	Cygnus Technologies	1 ml	EUR 322.00
Product category: Antibodies
Description: Anti-Human IgG3:HRP by Cygnus Technologies is available in Europe via Gentaur.

Anti-Human IgG4:HRP
IM154	Cygnus Technologies	1 ml	EUR 359.00
Product category: Antibodies
Description: Anti-Human IgG4:HRP by Cygnus Technologies is available in Europe via Gentaur.

Sheep, Anti-human IgG4
IM154U	Cygnus Technologies	1 ml	EUR 506.00
Product category: Antibodies
Description: Sheep, Anti-human IgG4 by Cygnus Technologies is available in Europe via Gentaur.

Anti-TRAIL (human) Antibody
A00466-2	BosterBio	100ul	EUR 417.00
Description: Rabbit Polyclonal TRAIL (human) Antibody. Validated in IF, IHC and tested in Human.

Anti-CCR4 (human) Antibody
A00755-1	BosterBio	200ug	EUR 397.00
Description: Goat Polyclonal CCR4 (human) Antibody. Validated in IF, IHC and tested in Human.

Anti-CCR10 (human) Antibody
A04731	BosterBio	200ug	EUR 397.00
Description: Goat Polyclonal CCR10 (human) Antibody. Validated in ELISA, IF, IHC and tested in Human.

Goat anti Human IgE
70R-IG015	Fitzgerald	1 mg	EUR 349.00
Description: Affinity purified Goat anti Human IgE antibody

Anti-human Rab5 antibody
A1511-50	Biovision		EUR 465.00

Anti-human TM9SF4 antibody
A1514-50	Biovision		EUR 446.00

Anti-human CD9 antibody
A1500-50	Biovision		EUR 465.00

Anti-human CD63 antibody
A1502-50	Biovision		EUR 490.00

Anti-human CD41 antibody
A1503-50	Biovision		EUR 468.00

Anti-human CD81 antibody
A1504-50	Biovision		EUR 468.00

Anti-human CD44 antibody
A1505-50	Biovision		EUR 468.00

Anti-human Alix Antibody
A1507-50	Biovision		EUR 501.00

Anti-human TM9SF4 antibody
A1508-50	Biovision		EUR 468.00

Anti-human Flotillin antibody
A1510-50	Biovision		EUR 501.00

Goat anti Human IgG
41C-CB0957	Fitzgerald	5 mg	EUR 346.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41C-CB0972	Fitzgerald	2 mg	EUR 264.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgM
41C-CB0993	Fitzgerald	1 mg	EUR 204.00
Description: Goat anti Human IgM secondary antibody

Goat anti Human IgG
41R-1026	Fitzgerald	2 mg	EUR 137.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1027	Fitzgerald	2 mg	EUR 186.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1028	Fitzgerald	1 mg	EUR 182.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1029	Fitzgerald	2 mg	EUR 186.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1030	Fitzgerald	1 mg	EUR 138.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1031	Fitzgerald	1 mg	EUR 186.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1032	Fitzgerald	1 mg	EUR 224.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1033	Fitzgerald	1 mg	EUR 246.00
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG
41R-1034	Fitzgerald	1 mg	EUR 186.00
Description: Goat anti Human IgG secondary antibody

Mouse anti Human IgM
41R-1586	Fitzgerald	100 ug	EUR 265.00
Description: Monoclonal Mouse anti Human IgM antibody

Goat anti Human IgE
40-IG10	Fitzgerald	1 ml	EUR 133.00
Description: Goat anti Human IgE antibody

Sheep anti Human IgE
40-IG11	Fitzgerald	1 ml	EUR 133.00
Description: Sheep anti Human IgE antibody

Rabbit anti Human IgG
40C-CB0943	Fitzgerald	1 mg	EUR 249.00
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG
40C-CB0950	Fitzgerald	5 mg	EUR 381.00
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG
40C-CB0971	Fitzgerald	2 mg	EUR 322.00
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgM
40C-CB9100	Fitzgerald	2 mg	EUR 283.00
Description: Rabbit anti Human IgM secondary antibody

Rabbit anti Human IgA
40C-CB9114	Fitzgerald	2 mg	EUR 259.00
Description: Rabbit anti Human IgA secondary antibody

Sheep anti Human IgM
40C-CR2023S	Fitzgerald	1 ml	EUR 241.00
Description: Sheep anti Human IgM antibody

Mouse anti Human IgE
40R-1000	Fitzgerald	100 ug	EUR 265.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgA
40R-1001	Fitzgerald	100 ug	EUR 265.00
Description: Mouse anti Human IgA antibody

Mouse anti Human IgG
40R-1003	Fitzgerald	100 ug	EUR 265.00
Description: Mouse anti Human IgG antibody

Mouse anti Human IgE
40R-1004	Fitzgerald	100 ug	EUR 265.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgM
40R-1005	Fitzgerald	100 ug	EUR 265.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgA
40R-1006	Fitzgerald	100 ug	EUR 265.00
Description: Mouse anti Human IgA antibody

Rat anti Human IgG1
40R-1007	Fitzgerald	100 ug	EUR 265.00
Description: Rat monoclonal Rat anti Human IgG1 antibody

Mouse anti Human IgA
40R-1011	Fitzgerald	500 ug	EUR 565.00
Description: Mouse anti Human IgA secondary antibody

Mouse anti Human IgA
40R-1012	Fitzgerald	500 ug	EUR 565.00
Description: Mouse anti Human IgA secondary antibody

Mouse anti Human IgG3
40R-1015	Fitzgerald	1 mg	EUR 414.00
Description: Mouse anti Human IgG3 antibody

Rat anti Human IgG
40R-1588	Fitzgerald	100 ug	EUR 265.00
Description: Rat monoclonal Rat anti Human IgG antibody

Goat anti Human IgG
41-XG56	Fitzgerald	1 mg	EUR 101.00
Description: Goat anti Human IgG secondary antibody

Mouse anti Human IgM
10C-CR2023M2	Fitzgerald	1 mg	EUR 154.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM
10C-CR2023M3	Fitzgerald	1 mg	EUR 154.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgA
10C-CR6043M1	Fitzgerald	1 mg	EUR 241.00
Description: Mouse anti Human IgA antibody

Mouse anti Human IgE
10C-CR6046M3	Fitzgerald	1 mg	EUR 165.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10C-CR6046M4	Fitzgerald	1 mg	EUR 165.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10C-CR6046M5	Fitzgerald	1 mg	EUR 165.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgG
10C-CR6047M1	Fitzgerald	1 mg	EUR 176.00
Description: Mouse anti Human IgG antibody

Mouse anti Human IgG
10C-CR6047M2	Fitzgerald	1 mg	EUR 176.00
Description: Mouse anti Human IgG antibody

Mouse anti Human IgA
10-7808	Fitzgerald	1 mg	EUR 336.00
Description: Mouse anti Human IgA antibody

Mouse anti Human IgA
10-I05A	Fitzgerald	1 mg	EUR 308.00
Description: Mouse anti Human IgA antibody

Mouse anti Human IgE
10-I104B	Fitzgerald	1 mg	EUR 336.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10-I104CC	Fitzgerald	1 mg	EUR 336.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10-I10A	Fitzgerald	1 mg	EUR 181.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10-I10C	Fitzgerald	1 mg	EUR 181.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10-I10E	Fitzgerald	1 mg	EUR 495.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgM
10-I20A	Fitzgerald	1 mg	EUR 191.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM
10-I20B	Fitzgerald	1 mg	EUR 295.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM
10-I20G	Fitzgerald	1 mg	EUR 295.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM
10-I20H	Fitzgerald	1 mg	EUR 187.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgG1
10-I21A	Fitzgerald	200 ug	EUR 220.00
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG2
10-I22A	Fitzgerald	200 ug	EUR 392.00
Description: Mouse anti Human IgG2 antibody

Mouse anti Human IgG3
10-I23A	Fitzgerald	200 ug	EUR 635.00
Description: Mouse anti Human IgG3 antibody

Mouse anti Human IgG4
10-I24A	Fitzgerald	200 ug	EUR 392.00
Description: Mouse anti Human IgG4 antibody

Mouse anti Human IgE
10-S9601MND1-M0	Fitzgerald	1 mg	EUR 219.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10-1025	Fitzgerald	1 mg	EUR 327.00
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE
10-1026	Fitzgerald	1 mg	EUR 327.00
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE
10-1027	Fitzgerald	1 mg	EUR 327.00
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE
10R-I104d	Fitzgerald	1 mg	EUR 447.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE
10R-I104e	Fitzgerald	1 mg	EUR 489.00
Description: Mouse anti Human IgE antibody

Mouse anti Human IgG1
10R-I110a	Fitzgerald	1 ml	EUR 554.00
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG1
10R-I127a	Fitzgerald	1 mg	EUR 489.00
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG2
10R-I128a	Fitzgerald	1 mg	EUR 489.00
Description: Mouse anti Human IgG2 antibody

Mouse anti Human IgG4
10R-I129a	Fitzgerald	1 mg	EUR 478.00
Description: Mouse anti Human IgG4 antibody

Mouse anti Human IgM
10R-I130a	Fitzgerald	1 mg	EUR 319.00
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM
10R-I130B	Fitzgerald	1 mg	EUR 300.00
Description: Mouse anti Human IgM antibody

Sheep anti Human IgA
20C-CR6043SP	Fitzgerald	1 ml	EUR 221.00
Description: Sheep anti Human IgA antibody

Sheep anti Human IgA
20C-CR6043SP-S	Fitzgerald	1 ml	EUR 221.00
Description: Sheep anti Human IgA antibody

Sheep anti Human IgA
20-1266	Fitzgerald	1ml	EUR 647.00
Description: Sheep polyclonal Human IgA secondary antibody

Sheep anti Human IgG
20-1377	Fitzgerald	1ml	EUR 4723.00
Description: Sheep polyclonal Human IgG secondary antibody