PCR versus qPCR Measurements on the Worldwide Scale for the Molecular Monitoring of Persistent Myeloid Leukemia

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A Simplified Quantitative Actual-Time PCR Assay for Monitoring SARS-CoV-2 Development in Cell Tradition

Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has contaminated hundreds of thousands inside just some months, inflicting extreme respiratory illness and mortality. Assays to watch SARS-CoV-2 development in vitro rely on time-consuming and dear RNA extraction steps, hampering progress in primary analysis and drug growth efforts. Right here, we developed a simplified quantitative real-time PCR assay that bypasses viral RNA extraction steps and might monitor SARS-CoV-2 development from a small quantity of cell tradition supernatants. As well as, we present that this method is definitely adaptable to quite a few different RNA and DNA viruses.

Utilizing this assay, we screened the actions of a lot of compounds that had been predicted to change SARS-CoV-2 entry and replication in addition to HIV-1-specific medication in a proof-of-concept examine. We discovered that E64D (inhibitor of endosomal proteases cathepsin B and L) and apilimod (endosomal trafficking inhibitor) potently decreased the quantity of SARS-CoV-2 RNA in cell tradition supernatants with minimal cytotoxicity.

Surprisingly, we discovered that the macropinocytosis inhibitor ethylisopropylamiloride (EIPA) equally decreased SARS-CoV-2 RNA ranges in supernatants, suggesting that entry may also be mediated by an alternate pathway. HIV-1-specific inhibitors nevirapine (a nonnucleoside reverse transcriptase inhibitor [NNRTI]), amprenavir (a protease inhibitor), and allosteric integrase inhibitor 2 (ALLINI-2) modestly inhibited SARS-CoV-2 replication, albeit the 50% inhibitory focus (IC50) values had been a lot increased than that required for HIV-1.

Taking the info collectively, this simplified assay will expedite primary SARS-CoV-2 analysis, be amenable to mid-throughput screening assays (i.e., drug, CRISPR, small interfering RNA [siRNA], and so forth.), and be relevant to a broad variety of RNA and DNA viruses.

IMPORTANCE Extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of the coronavirus illness 2019 (COVID-19) pandemic, is constant to trigger immense respiratory illness and social and financial disruptions.

Standard assays that monitor SARS-CoV-2 development in cell tradition depend on pricey and time-consuming RNA extraction procedures, hampering progress in primary SARS-CoV-2 analysis and growth of efficient therapeutics. Right here, we developed a easy quantitative real-time PCR assay to monitor SARS-CoV-2 development in cell tradition supernatants that doesn’t necessitate RNA extraction and that’s as correct and delicate as present strategies. In a proof-of-concept display screen, we discovered that E64D, apilimod, EIPA, and remdesivir can considerably impede SARS-Cov-2 replication, offering novel perception into viral entry and replication mechanisms.

As well as, we present that this method is definitely adaptable to quite a few different RNA and DNA viruses. This simplified assay will undoubtedly expedite primary SARS-CoV-2 and virology analysis and be amenable to make use of in drug screening platforms to establish therapeutics in opposition to SARS-CoV-2.

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learninglabs

Rabbit Anti-Human alpha-Tubulin IgG

TUBA13-A 100 ul
EUR 482

Rabbit Anti-Human Fodrin-alpha (spectrin-alpha) IgG, aff pure

FOD11-A 100 ug
EUR 482

Rabbit Anti-Human Estrogen receptor alpha (ER-alpha) peptide IgG

ERA12-A 100 ug
EUR 482

Chicken Anti-Human alpha 1 anti-trypsin IgG/Y

A1AT12-A 100 ul
EUR 482

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 280

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Rabbit Anti-Human p73 alpha antiserum # 2

P73A12-S 100 ul
EUR 457

Rabbit Anti-Human Synuclein-alpha antiserum # 1

SYN11-S 100 ul
EUR 457

Goat Anti-human Chorionic Gonadotropin Alpha (hCG-Alpha) IgG aff pure

HCGA13-A 1 mg
EUR 286

Monoclonal anti-human Alpha (FSH-Alpha), IgG clone 3 aff pure

FSHA12-M 1 mg
EUR 482

Goat Anti-Human Lutenizing Hormone Alpha (LH-Alpha) IgG aff pure

LHA13-A 1 mg
EUR 286

Rabbit Anti-human Topoisomerase II alpha (TOP2 alpha) IgG, aff pure

TOP2A11-A 100 ug
EUR 482

Rabbit Anti-Mouse Neprilysin-alpha (NEPL-alpha) antiserum

NEPLA11-S 100 ul
EUR 457

Monoclonal Anti-Human Chorionic Gonadotropin Alpha (hCG-alpha) IgG #1, aff pure

HCGA11-M 1 mg
EUR 286

Monoclonal Anti-human Chronionic Gonadotropin Alpha (hCG-Alpha) IgG #2, caff pure

HCGA12-M 1 mg
EUR 286

Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #2

RELMA12-S 100 ul
EUR 457

Goat Anti-human Alpha fetoprotein (AFP) IgG, pure

AFP13-A 1 mg
EUR 651

Rabbit Anti-Human p73 alpha/beta antiserum # 1

P73A11-S 100 ul
EUR 457

Rabbit Anti-Human alpha-Tubulin IgG-Biotin Conjugate

TUBA13-BTN 100 ul
EUR 469

Rabbit Anti-Human alpha-Tubulin IgG-HRP Conjugate

TUBA13-HRP 100 ul
EUR 432

Rabbit Anti-Rat Glutathione Transferase alpha (GST-alpha) antiserum

GSTA11-S 100 ul
EUR 457

Monoclonal anti-human Lutenizing Hormone Alpha (LH-Alpha) IgG, clone 2 aff pure

LHA12-M 1 mg
EUR 286

Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #1 aff. Pure

TNFA11-M 100 ug
EUR 482

Monoclonal Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure

TNFA12-M 100 ug
EUR 482

Rabbit Anti-Mouse Neprilysin-alpha (NEPL-alpha) IgG (aff pure)

NEPLA11-A 100 ug
EUR 482

Goat Anti-Ferret IgA (alpha), unlabeled

70519 0.5 mg
EUR 213

Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 1)

AFP11-M 1 mg
EUR 482

Mouse Monoclonal Anti-human Alpha fetoprotein (AFP) (clone 2)

AFP12-M 1 mg
EUR 482

Rabbit Anti-Human HE2 alpha IgG #1, aff pure

HE2A11-A 100 ug
EUR 482

Chicken Anti-Human Alpha 2 Macroglobulin (A2M) IgG/Y

A2MG13-A 100 ul
EUR 482

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, unlabeled

A1AT11-A 100 ul
EUR 408

Goat Anti-Human IgA (alpha chain sp.), aff pure

10019 0.5 mg
EUR 202

Rabbit Anti-Human p73 alpha/ IgG # 2, aff pure

P73A12-A 100 ug
EUR 482

Rabbit Anti-Human Synuclein-alpha IgG # 1, aff pure

SYN11-A 100 ug
EUR 482

Monoclonal anti-human Follicle Stimulating Hormone Alpha (FSH-Alpha), IgG clone 2 aff pure

FSHA11-M 1 mg
EUR 482

Rabbit Anti-Human Resistin-Like protein alpha (RELM-alpha/FIZZ1) IgG #2, aff. Pure

RELMA12-A 100 ug
EUR 482

Goat Anti-Human Tumor Necrosis Factor-Alpha (TNF-alpha) IgG #2, aff. Pure (neutralzing)

TNFA13-A 100 ul
EUR 482

Goat Anti-Follicle Stimulating Hormone Alpha (FSH-Alpha) IgG aff pure

FSH13-A 1 mg
EUR 482

monoclonal Anti-Rat Resistin-Like protein alpha (RELM-alpha/FIZZ1) ascites

RELMA14-M 100 ul
EUR 482

Rabbit Anti-Human Core-binding factor alpha (CBFA1) antiserum #1

CBFA11-S 100 ul
EUR 457

Sheep Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure

ACT11-A 100 ug
EUR 408

Monoclonal Anti-Human alpha 1 antichymotrypsin (ACT) IgG, aff pure

ACT12-M 100 ug
EUR 482

Rabbit Anti-Human Alpha Defensin 4 (NP-4) antiserum #1

HDEFA41-S 100 ul
EUR 457

Rabbit Anti-Human Alpha Defensin 5 (NP-5),antiserum #1

HDEFA51-S 100 ul
EUR 457

Rabbit Anti-Human Alpha Defensin 6 (NP-6) antiserum #1

HDEFA61-S 100 ul
EUR 457

Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) Antiserum #1

GAA11-S 100 ul
EUR 457

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, unlabeled

A2MG11-A 100 ul
EUR 347

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, Biotin conjugate

A1AT11-BT 100 ul
EUR 408

Goat Anti-Human Alpha 1-Antitrypsin (A1AT) IgG, HRP conjugate

A1AT11-HRP 100 ul
EUR 408

Goat Anti-Human IgA (alpha chain sp.) IgG-Biotin Conjugate

10040 0.5 mg
EUR 202

Goat Anti-Human IgA (alpha chain sp.)-Alk. Phosphatase conjugate

10050 0.5 ml
EUR 225

Human Anti-Alpha Fodrin IgG ELISA kit, 96 tests, Quantitative

3300-160-AFG 1 kit
EUR 590

Rabbit Anti-Human p73 alpha/beta IgG # 1, aff pure

P73A11-A 100 ug
EUR 482

Mouse Monoclonal Anti-Human Synuclein-alpha protein IgG, aff pure

SYNRP-84816-M 100 ug
EUR 482

Goat Anti-Ferret IgA (alpha)-HRP conjugate

70520 0.5 ml
EUR 238

Rabbit Anti-C. perfringens alpha toxin antiserum

CPA11-S 100 ul
EUR 457

Rabbit Anti-Rat Synuclein-alpha antiserum # 2

SYN12-S 100 ul
EUR 457

Rabbit Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) antiserum #1

RELMA11-S 100 ul
EUR 457

Rat monoclonal Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) ascites

RELMA13-M 100 ul
EUR 482

Recombinant (E.Coli) Human p38 alpha/SAPK2 alpha

RP-675 1 ug
EUR 286

Rabbit Anti-Human IL-2 receptor alpha, soluble (IL2RsA/CD25) IgG

CD251-A 100 ul
EUR 482

Monoclonal Anti-Human/mouse/rat HSP90 alpha/beta IgG, aff pure

HSP901-M 100 ul
EUR 482

Rabbit Anti-Human GABAa Receptor alpha 2 (GAA2) IgG, aff. Pure

GAA21-A 100 ug
EUR 482

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, Biotin conjugate

A2MG11-BT 100 ul
EUR 286

Goat Anti-human Alpha 2-Macroglobulin (A2M) protein IgG, HRP conjugate

A2MG11-HRP 100 ul
EUR 286

Chicken Anti-Human Alpha 1 acid glycoprotein (A1AG) IgG/Y, unlabeled

A1AG12-A 100 ul
EUR 482

Monoclonal Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein, ascites

A1AG12-M 100 ul
EUR 482

Monoclonal Anti-Human Lutenizing Hormone (LH), alpha chain IgG, aff pure

LHA14-M 1 mg
EUR 286

Goat Anti-Rat IgA (alpha chain)-HRP conjugate

50020 0.5 ml
EUR 225

Goat Anti-Rat IgA (alpha chain)-Biotin conjugate

50040 0.5 ml
EUR 225

Rabbit Anti-Goat IgA (alpha chain)-HRP conjugate

30120 0.5 ml
EUR 213

Rabbit Anti-Goat IgA (alpha chain)-FITC conjugate

30130 0.5 ml
EUR 213

Goat Anti-Rabbit IgA (alpha chain)-HRP conjugate

20029 0.5 ml
EUR 263

Goat Anti-Chicken IgA (alpha chain)-HRP conjugate

60220 0.5 ml
EUR 213

Goat Anti-Chicken IgA (alpha chain)-FITC Conjugate

60230 0.5 ml
EUR 213

Goat Anti-Chicken IgA (alpha chain)-biotin conjugate

60240 0.5 ml
EUR 225

Goat Anti-Monkey IgA (alpha-chain specific), unlabeled

70043 0.5 mg
EUR 225

Monoclonal Anti-Bovine S100 alpha ab # 2, ascites

S1005-M 100 ul
EUR 482

Human Fodrin-alpha (spectrin-alpha) control/blocking peptide

FOD11-P 100 ug
EUR 164

Recombinant Human Alpha-Synuclein

RP-847 20 ug
EUR 164

Rabbit Anti-Mouse Resistin-Like protein alpha (RELM-alpha/FIZZ1) IgG#1, aff. pure

RELMA11-A 100 ug
EUR 482

Rabbit Anti-Human/mouse/rat HSP90 alpha (hsp90AB1) peptide IgG, aff pure

HSP903-A 100 ul
EUR 482

Rabbit Anti-Human Alpha Defensin 4 (NP-4), IgG #1, aff pure

HDEFA41-A 100 ug
EUR 482

Rabbit Anti-Human Alpha Defensin 5 (NP-5),, IgG #1, aff pure

HDEFA51-A 100 ug
EUR 482

Rabbit Anti-Human Alpha Defensin 6 (NP-6) IgG #1, aff pure

HDEFA61-A 100 ug
EUR 482

Monoclonal Anti-Human Fetuin A (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG

FETA13-M 100 ug
EUR 482

Sheep Anti-Human Serum Fetuin (Alpha-2 HS-Glycoprotein, AHSG, A2HS) IgG

FETA15-A 100 ul
EUR 408

Rabbit Anti-Human GABAa Receptor alpha 1 (GAA1) IgG #1, aff pure

GAA11-A 100 ug
EUR 482

Rabbit Anti-Human/Rat GABAa Receptor alpha 3 (GAA3) IgG, aff pure

GAA31-A 100 ug
EUR 482

Rabbit Anti-Human Alpha 1-Acid Glycoprotein (A1-AGP) protein IgG, unlabeled

A1AG11-A 100 ul
EUR 408

Rabbit Anti-Human interleukin 13 receptor, alpha 2 (IL13RA2) IgG (aff pure)

AB-23040-A 100ug
EUR 482

Rabbit Anti-Human PUMA-alpha (p53-upregulated modulator of apoptosis) antiserum #1

PUMA11-S 100 ul
EUR 457

Rabbit Anti-Human PUMA-alpha (p53-upregulated modulator of apoptosis) antiserum #1

PUMA12-S 100 ul
EUR 457

Mouse Monoclonal Anti-Human Luteinizing Hormone (LH), alpha chain IgG, aff pure

LH13-M 1 mg
EUR 286

Monoclonal Anti-human Lutenizing Hormone (LH- Alpha) IgG, clone 1 aff pure

LHA11-M 1 mg
EUR 286

human Topoisomerase II alpha (TOP2 alpha) Control/blocking peptide

TOP2A11-P 100 ug
EUR 164

Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma)-biotinylated

ACTB24-BTN 50 ul
EUR 408

Comparability of Droplet Digital PCR versus qPCR Measurements on the Worldwide Scale for the Molecular Monitoring of Persistent Myeloid Leukemia Sufferers

Background: BCR-ABL1/ABL1 p210 measurement by quantitative polymerase chain response (qPCR) is used worldwide to watch the molecular response in persistent myeloid leukemia (CML) sufferers. Droplet digital polymerase chain response (ddPCR) appears to indicate a higher sensitivity than qPCR, most likely because of the excessive variety of replicates analyzed in ddPCR for the comparability. Moreover, in a just lately printed comparability, ddPCR measurements weren’t adequately reworked into Worldwide Scale (IS).

Technique: We’ve analyzed 50 CML sufferers and ten non-CML donors in parallel by qPCR and ddPCR. To the very best of our data, that is the primary examine evaluating each methods below related circumstances, with BCR-ABL1/ABL1 measurements carried out through each methods reworked into IS.

Outcomes: Qualitative and quantitative comparisons confirmed wonderful outcomes. The qualitative correlation confirmed a Kappa index of 0.94 (95% confidence interval [CI] 0.90-0.98) (P < 0.001). Within the quantitative comparability, absolutely the intra-class correlation coefficient was 0.868 (95% CI 0.734-0.937; P < 0.001), and Lin’s concordance correlation coefficient was 0.863.

The Passing-Bablock check indicated a slight proportional distinction between qPCR and ddPCR. A quantitative and qualitative subanalysis together with 40 sufferers with a molecular response of three.Zero or deeper confirmed related ends in each check. As well as, the proportional distinction within the Passing-Bablock check disappeared. There have been no variations within the sensitivity for BCR-ABL1 detection between qPCR and ddPCR (McNemar check, P = 0.5).

Conclusions: In conclusion, our outcomes present superb quantitative and qualitative correlations between BCR-ABL1/ABL1 p210 outcomes obtained by qPCR and by ddPCR and make sure earlier scarce information concerning the shortage of a rise in sensitivity of ddPCR over qPCR on this setting.

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 280

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

anti-human CCR1

20R-3028 200 ug
EUR 587
Description: Goat anti-human CCR1 antibody

anti-human RecQL4

AR05-PA0007 100 ul
EUR 334
Description: Rabbit polyclonal to human RecQL4

Anti-Human IgG

DB-173-0.1 100 μl
EUR 212
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-173-0.2 200 μl
EUR 298
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-173-0.5 500 μl
EUR 384
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-173-1 1 ml
EUR 613
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-173-RTU-15 15 ml
EUR 355
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB-173-RTU-7 7 ml
EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB-174-0.1 100 μl
EUR 212
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-174-0.2 200 μl
EUR 298
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-174-0.5 500 μl
EUR 384
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-174-1 1 ml
EUR 613
Description: rabbit monospecific clonal antibodies for ihc-p application; concentrated

Anti-Human IgG

DB-174-RTU-15 15 ml
EUR 355
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB-174-RTU-7 7 ml
EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB173RTU-15 15 ml
EUR 355
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB173RTU-7 7 ml
EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB174RTU-15 15 ml
EUR 355
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Anti-Human IgG

DB174RTU-7 7 ml
EUR 231
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

anti-human Albumin

LF-PA10001 100 ug
EUR 403
Description: Rabbit polyclonal to human Albumin

Human anti AMPH(anti amphiphysin) ELISA Kit

EH2621 96T
EUR 524.1
  • Detection range: 31.25-2000 pg/ml
  • Uniprot ID: P49418
  • Alias: anti-AMPH
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens;Sensitivity: 18.75pg/ml

Human anti-TPO(anti-thrombopoietin) ELISA Kit

EH4147 96T
EUR 524.1
  • Detection range: 1.563-100 ng/ml
  • Alias: anti-TPO
Description: Method of detection: Sandwich ELISA, Double Antigen;Reacts with: Homo sapiens ;Sensitivity: 0.938 ng/ml

Goat anti-Human anti-thrombin polyclonal antibody

CABT-L487 500ug
EUR 715

Sheep anti-Human anti-thrombin polyclonal antibody

CABT-L488 500ug
EUR 663

Sheep anti-Human anti-thrombin polyclonal antibody

CABT-L489 100ug
EUR 663

Sheep anti-Human anti-thrombin polyclonal antibody

CABT-L490 100ug
EUR 663

Sheep anti-Human anti-thrombin polyclonal antibody

CABT-L491 100ug
EUR 663

Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *

V100125 50 ug
EUR 306
  • R-phrase:
  • H-Phrase:
  • Symbol for dangerous compounds:
  • UNSPEC Code:

Anti-Procalcitonin antibody *Mouse anti-human, monoclonal *

V100126 1 mg
EUR 393
  • R-phrase:
  • H-Phrase:
  • Symbol for dangerous compounds:
  • UNSPEC Code:

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Int
  • Show more
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

4-AEA465Hu
  • EUR 5698.00
  • EUR 3011.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Anti-Sperm Antibody elisa. Alternative names of the recognized antigen: Anti-Spermatozoa Antibodies
  • Sperm Antibodies
  • Antisperm Antibodies
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)

ELK8071 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate soluti
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Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Sheep anti Human IgA

20C-CR6043SP 1 ml
EUR 221
Description: Sheep anti Human IgA antibody

Sheep anti Human IgA

20C-CR6043SP-S 1 ml
EUR 221
Description: Sheep anti Human IgA antibody

Goat anti Human IgM

70-B9022GA00-A0 5 mg
EUR 192
Description: Goat anti Human IgM antibody

Goat anti Human IgG

70C-CR6047GAP 1 mg
EUR 149
Description: Affinity purified Goat anti Human IgG antibody

Anti-CCR10 (human) Antibody

A04731 200ug
EUR 397
Description: Goat Polyclonal CCR10 (human) Antibody. Validated in ELISA, IF, IHC and tested in Human.

Mouse anti Human IgE

10R-I104d 1 mg
EUR 447
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10R-I104e 1 mg
EUR 489
Description: Mouse anti Human IgE antibody

Mouse anti Human IgG1

10R-I110a 1 ml
EUR 554
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG1

10R-I127a 1 mg
EUR 489
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG2

10R-I128a 1 mg
EUR 489
Description: Mouse anti Human IgG2 antibody

Mouse anti Human IgG4

10R-I129a 1 mg
EUR 478
Description: Mouse anti Human IgG4 antibody

Mouse anti Human IgM

10R-I130a 1 mg
EUR 319
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10R-I130B 1 mg
EUR 300
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10C-CR2023M2 1 mg
EUR 154
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10C-CR2023M3 1 mg
EUR 154
Description: Mouse anti Human IgM antibody

Mouse anti Human IgA

10C-CR6043M1 1 mg
EUR 241
Description: Mouse anti Human IgA antibody

Mouse anti Human IgE

10C-CR6046M3 1 mg
EUR 165
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10C-CR6046M4 1 mg
EUR 165
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10C-CR6046M5 1 mg
EUR 165
Description: Mouse anti Human IgE antibody

Mouse anti Human IgG

10C-CR6047M1 1 mg
EUR 176
Description: Mouse anti Human IgG antibody

Mouse anti Human IgG

10C-CR6047M2 1 mg
EUR 176
Description: Mouse anti Human IgG antibody

Mouse anti Human IgA

10-7808 1 mg
EUR 336
Description: Mouse anti Human IgA antibody

Mouse anti Human IgA

10-I05A 1 mg
EUR 308
Description: Mouse anti Human IgA antibody

Mouse anti Human IgE

10-I104B 1 mg
EUR 336
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I104CC 1 mg
EUR 336
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I10A 1 mg
EUR 181
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I10C 1 mg
EUR 181
Description: Mouse anti Human IgE antibody

Mouse anti Human IgE

10-I10E 1 mg
EUR 495
Description: Mouse anti Human IgE antibody

Mouse anti Human IgM

10-I20A 1 mg
EUR 191
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10-I20B 1 mg
EUR 295
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10-I20G 1 mg
EUR 295
Description: Mouse anti Human IgM antibody

Mouse anti Human IgM

10-I20H 1 mg
EUR 187
Description: Mouse anti Human IgM antibody

Mouse anti Human IgG1

10-I21A 200 ug
EUR 220
Description: Mouse anti Human IgG1 antibody

Mouse anti Human IgG2

10-I22A 200 ug
EUR 392
Description: Mouse anti Human IgG2 antibody

Mouse anti Human IgG3

10-I23A 200 ug
EUR 635
Description: Mouse anti Human IgG3 antibody

Mouse anti Human IgG4

10-I24A 200 ug
EUR 392
Description: Mouse anti Human IgG4 antibody

Mouse anti Human IgE

10-S9601MND1-M0 1 mg
EUR 219
Description: Mouse anti Human IgE antibody

Sheep anti Human IgA

20-1266 1ml
EUR 647
Description: Sheep polyclonal Human IgA secondary antibody

Sheep anti Human IgG

20-1377 1ml
EUR 4723
Description: Sheep polyclonal Human IgG secondary antibody

Goat anti Human IgA

20-B9001G000-S0 10 ml
EUR 133
Description: Goat anti Human IgA antibody

Goat anti Human IgM

20-B9022G000-S2 10 ml
EUR 133
Description: Goat anti Human IgM antibody

Rabbit anti Human IgE

20-IR77 1 ml
EUR 192
Description: Rabbit anti Human IgE antibody

Goat anti Human IgA

20-S11030GND1-D 5 mg
EUR 192
Description: Goat anti Human IgA antibody

Goat anti Human IgA

20-S1103GND1-D0 10 ml
EUR 192
Description: Goat anti Human IgA antibody

Goat anti Human IgA

20-S1111G000-S4 10 ml
EUR 133
Description: Goat anti Human IgA antibody

Goat anti Human IgA

20-S1111G000-V0 10 ml
EUR 133
Description: Goat anti Human IgA antibody

Goat anti Human IgA

20-S1111GOOO-S4 10 ml
EUR 133
Description: Goat anti Human IgA antibody

Goat anti Human IgM

20-S1303GND1-D0 10 ml
EUR 192
Description: Goat anti Human IgM antibody

Goat anti Human IgM

20-S1304GA01 5 ml
EUR 138
Description: Goat anti Human IgM antibody

Goat anti Human IgM

20-S1311G000-S4 10 ml
EUR 133
Description: Goat anti Human IgM antibody

Goat anti Human IgM

20-S1311G000-V0 10 ml
EUR 111
Description: Goat anti Human IgM antibody

Goat anti Human IgM

20-S1311G001-S4 10 ml
EUR 133
Description: Goat anti Human IgM antibody

Goat anti Human IgE

20-S2001G000-S4 10 ml
EUR 127
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2001G000-V0 10 ml
EUR 133
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2001G000-VO 10 ml
EUR 133
Description: Goat anti Human IgE antibody

Goat anti Human IgE

20-S2003GND1-D0 10 ml
EUR 192
Description: Goat anti Human IgE antibody

Goat anti Human IgM

20-S4113GN00-D4 10 ml
EUR 192
Description: Goat anti Human IgM antibody

Goat anti Human IgM

20-S5170GND1-D0 10 ml
EUR 192
Description: Goat anti Human IgM antibody

Goat anti Human IgA

20-S5491G000-S4 10 ml
EUR 133
Description: Goat anti Human IgA antibody

Mouse anti Human IgE

10-1025 1 mg
EUR 327
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE

10-1026 1 mg
EUR 327
Description: Mouse monoclonal Mouse anti Human IgE antibody

Mouse anti Human IgE

10-1027 1 mg
EUR 327
Description: Mouse monoclonal Mouse anti Human IgE antibody

Rabbit anti Human IgG

40C-CB0943 1 mg
EUR 249
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG

40C-CB0950 5 mg
EUR 381
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgG

40C-CB0971 2 mg
EUR 322
Description: Rabbit anti Human IgG secondary antibody

Rabbit anti Human IgM

40C-CB9100 2 mg
EUR 283
Description: Rabbit anti Human IgM secondary antibody

Rabbit anti Human IgA

40C-CB9114 2 mg
EUR 259
Description: Rabbit anti Human IgA secondary antibody

Sheep anti Human IgM

40C-CR2023S 1 ml
EUR 241
Description: Sheep anti Human IgM antibody

Mouse anti Human IgE

40R-1000 100 ug
EUR 265
Description: Mouse anti Human IgE antibody

Mouse anti Human IgA

40R-1001 100 ug
EUR 265
Description: Mouse anti Human IgA antibody

Mouse anti Human IgG

40R-1003 100 ug
EUR 265
Description: Mouse anti Human IgG antibody

Mouse anti Human IgE

40R-1004 100 ug
EUR 265
Description: Mouse anti Human IgE antibody

Mouse anti Human IgM

40R-1005 100 ug
EUR 265
Description: Mouse anti Human IgM antibody

Mouse anti Human IgA

40R-1006 100 ug
EUR 265
Description: Mouse anti Human IgA antibody

Rat anti Human IgG1

40R-1007 100 ug
EUR 265
Description: Rat monoclonal Rat anti Human IgG1 antibody

Mouse anti Human IgA

40R-1011 500 ug
EUR 565
Description: Mouse anti Human IgA secondary antibody

Mouse anti Human IgA

40R-1012 500 ug
EUR 565
Description: Mouse anti Human IgA secondary antibody

Mouse anti Human IgG3

40R-1015 1 mg
EUR 414
Description: Mouse anti Human IgG3 antibody

Rat anti Human IgG

40R-1588 100 ug
EUR 265
Description: Rat monoclonal Rat anti Human IgG antibody

Goat anti Human IgG

41-XG56 1 mg
EUR 101
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41C-CB0957 5 mg
EUR 346
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41C-CB0972 2 mg
EUR 264
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgM

41C-CB0993 1 mg
EUR 204
Description: Goat anti Human IgM secondary antibody

Goat anti Human IgG

41R-1026 2 mg
EUR 137
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1027 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1028 1 mg
EUR 182
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1029 2 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1030 1 mg
EUR 138
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1031 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1032 1 mg
EUR 224
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1033 1 mg
EUR 246
Description: Goat anti Human IgG secondary antibody

Goat anti Human IgG

41R-1034 1 mg
EUR 186
Description: Goat anti Human IgG secondary antibody

Mouse anti Human IgM

41R-1586 100 ug
EUR 265
Description: Monoclonal Mouse anti Human IgM antibody

Goat anti Human IgE

40-IG10 1 ml
EUR 133
Description: Goat anti Human IgE antibody

Sheep anti Human IgE

40-IG11 1 ml
EUR 133
Description: Sheep anti Human IgE antibody

Anti-CCR4 (human) Antibody

A00755-1 200ug
EUR 397
Description: Goat Polyclonal CCR4 (human) Antibody. Validated in IF, IHC and tested in Human.

Goat anti Human IgE

70R-IG015 1 mg
EUR 349
Description: Affinity purified Goat anti Human IgE antibody

Anti-TRAIL (human) Antibody

A00466-2 100ul
EUR 417
Description: Rabbit Polyclonal TRAIL (human) Antibody. Validated in IF, IHC and tested in Human.

Rabbit Anti Human IgG

E61I00101 100ug
EUR 343

Goat Anti Human IgG

E61I00201 1mg
EUR 274

Goat Anti Human IgM

E61I00401 100ug
EUR 343

Mouse Anti Human IgM

E61I00402 100ug
EUR 343

Goat anti human IgA

E61I01201 1mg
EUR 611

Mouse anti human IgA

E61I02101 100ug
EUR 343

Goat anti human IgA

E61I02102 100ug
EUR 343

Mouse anti human IgE

E61I02201 100ug
EUR 343

Anti-human CD47 mAb

CM096-100ug 100ug
EUR 347

Anti-human CD47 mAb

CM096-25ug 25ug
EUR 202

Anti-human CD45 mAb

CM100-100ug 100ug
EUR 122

Anti-human CD45 mAb

CM100-25ug 25ug
EUR 86

Goat Anti-Human IgA

C020242-1mg 1mg
EUR 227

Goat Anti-Human IgA

C020242-50mg 50mg
EUR 2635

Anti-human CD9 antibody

A1500-50
EUR 465

Anti-human CD63 antibody

A1502-50
EUR 490

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